Diwan M, Misra A, Khar R K, Talwar G P
International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Vaccine. 1997 Dec;15(17-18):1867-71. doi: 10.1016/s0264-410x(97)00137-0.
Cross-linked dextran beads were used as a carrier for development of a 'single-contact' vaccine delivery system. Diphtheria toxoid (DT) was covalently coupled to dextran beads (DEX-DT conjugate). The conjugate was immunoreactive with antibodies raised against native DT. Immunization of rats with DEX-DT conjugate generated on average 24 times higher antibody titres against diphtheria toxoid than immunization with the conventional DT absorbed on alum. The immune response was sustained for 9 months, with gradual decline of antibody titres thereafter. Significant antibody titres were measurable in rats after 1 year of immunization. DEX-DT had neither acute nor long-term adverse effects on the health of animals, as evidenced by local reactions, general behaviour, food intake, body weight gain and survival compared with controls.
交联葡聚糖珠用作开发“单次接触”疫苗递送系统的载体。白喉类毒素（DT）与葡聚糖珠共价偶联（DEX-DT偶联物）。该偶联物与针对天然DT产生的抗体具有免疫反应性。用DEX-DT偶联物免疫大鼠产生的抗白喉类毒素抗体滴度平均比用吸附在明矾上的传统DT免疫高24倍。免疫反应持续9个月，此后抗体滴度逐渐下降。免疫1年后在大鼠中可检测到显著的抗体滴度。与对照组相比，DEX-DT对动物健康既无急性也无长期不良影响，这通过局部反应、一般行为、食物摄入量、体重增加和存活率得到证明。
