Honkakoski P, Negishi M
Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
J Biochem Mol Toxicol. 1998;12(1):3-9. doi: 10.1002/(sici)1099-0461(1998)12:1<3::aid-jbt2>3.0.co;2-p.
This article reviews recent progress in characterizing cis-acting DNA elements of the phenobarbital-inducible CYP2B genes. Whereas proximal DNA elements such as the C/EBP binding site regulate basal transcription activity, phenobarbital-responsive enhancer activity is governed by the distal element (designated phenobarbital-responsive enhancer module, PBREM) residing about -2.3 kbp upstream from the transcription start site. Proximal elements are not required to enhance the phenobarbital-inducible transcription, since the PBREM can confer the inducibility to several heterologous promoters. Repression of the basal transcription by a negative element upstream of the -0.8 kbp region, however, may be necessary for the proper regulation of the CYP2B genes.
本文综述了在表征苯巴比妥诱导型CYP2B基因的顺式作用DNA元件方面的最新进展。近端DNA元件如C/EBP结合位点调节基础转录活性，而苯巴比妥反应性增强子活性则由位于转录起始位点上游约-2.3 kbp处的远端元件（称为苯巴比妥反应性增强子模块，PBREM）控制。近端元件对于增强苯巴比妥诱导的转录并非必需，因为PBREM可赋予几种异源启动子诱导性。然而，-0.8 kbp区域上游的负性元件对基础转录的抑制可能对于CYP2B基因的正常调控是必要的。
