Toyabe S, Iiai T, Fukuda M, Kawamura T, Suzuki S, Uchiyama M, Abo T
Department of Immunology, Niigata University School of Medicine, Japan.
Immunology. 1997 Oct;92(2):201-5. doi: 10.1046/j.1365-2567.1997.00323.x.
The existence of nicotinic acetylcholine receptors (nAChR) on lymphocytes remains controversial. We attempted to show the existence of nAChR on murine lymphocytes. The intraperitoneal injection of nicotine induced the lymphocytosis in the spleen on day 3. Although freshly isolated lymphocytes bound small quantities of fluorescein isothiocyanate (FITC)-conjugated alpha-bungarotoxin (alpha BuTx), they began to bind alpha BuTx after incubation in medium. In contrast to granulocytes, various lymphocyte subsets obtained from various lymphoid organs were found to bind alpha BuTx. Affinity purification of alpha BuTx-binding protein revealed that lymphocytes expressed the same nAChR molecules as those of muscle. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that lymphocytes expressed the alpha-subunit mRNA of nAChR. These results suggest that lymphocytes carry nAChR on the surface and are stimulated directly via their nAChR by parasympathetic nerve stimuli.
淋巴细胞上烟碱型乙酰胆碱受体（nAChR）的存在仍存在争议。我们试图证明小鼠淋巴细胞上存在nAChR。腹腔注射尼古丁在第3天可诱导脾脏淋巴细胞增多。虽然新鲜分离的淋巴细胞仅结合少量异硫氰酸荧光素（FITC）偶联的α-银环蛇毒素（α-BuTx），但在培养基中孵育后它们开始结合α-BuTx。与粒细胞不同，从各种淋巴器官获得的各种淋巴细胞亚群均能结合α-BuTx。对α-BuTx结合蛋白进行亲和纯化显示，淋巴细胞表达的nAChR分子与肌肉中的相同。逆转录聚合酶链反应（RT-PCR）分析表明，淋巴细胞表达nAChR的α亚基mRNA。这些结果表明，淋巴细胞表面带有nAChR，并可通过副交感神经刺激经其nAChR直接受到刺激。
