Holzinger A, Meindl U
Institute for Plant Physiology, University of Salzburg, Austria.
Cell Motil Cytoskeleton. 1997;38(4):365-72. doi: 10.1002/(SICI)1097-0169(1997)38:4<365::AID-CM6>3.0.CO;2-2.
Jasplakinolide, a naturally occurring cyclodepsipeptide from the marine sponge Jaspis sp., known to induce actin polymerization and stabilization in vitro, markedly influences the morphogenetic process in the green alga Micrasterias when used in concentrations higher than 3 microM. Development of Micrasterias is inhibited or strongly retarded, malformations occur, and large vacuoles are formed. At the ultrastructural level, dense abnormal accumulations of filamentous structures have been found indicating actin filament polymerizing activities of the drug in situ. Moreover, displacement of organelles and aggregations of endoplasmic reticulum (ER) cisternae have been observed. Microtubule (MT) arrangement and MT-dependent processes remain undisturbed. Cells allowed to recover from jasplakinolide treatment continue their growth but show severe changes in the cell pattern and displacement of organelles, suggesting that even after removal of the drug, some basic features for the morphogenetic process remain altered. Jasplakinolide might be a useful tool for investigations on actin-dependent processes in the future.
茉莉酮酸内酯是一种从海洋海绵Jaspis sp.中天然存在的环缩肽，已知其在体外可诱导肌动蛋白聚合和稳定，当以高于3 microM的浓度使用时，会显著影响绿藻微星鼓藻的形态发生过程。微星鼓藻的发育受到抑制或严重阻碍，出现畸形，并形成大液泡。在超微结构水平上，已发现丝状结构密集异常积累，表明该药物在原位具有肌动蛋白丝聚合活性。此外，还观察到细胞器移位和内质网（ER）池聚集。微管（MT）排列和依赖MT的过程未受干扰。从茉莉酮酸内酯处理中恢复的细胞继续生长，但细胞模式出现严重变化且细胞器移位，这表明即使去除药物后，形态发生过程的一些基本特征仍发生了改变。茉莉酮酸内酯可能是未来研究肌动蛋白依赖性过程的有用工具。
