Chang B V, Wu W B, Yuan S Y
Department of Microbiology, Soochow University Taipei, Taiwan, Republic of China.
Chemosphere. 1997 Dec;35(12):2807-15. doi: 10.1016/s0045-6535(97)00281-6.
Pseudomonas sp. D8 strain, which has the potential to utilize toluene as a sole carbon source, was isolated. At a concentration of 100 mg/l, this strain was found to efficiently degrade toluene and benzene (both individually and in mixture) in culture medium at 30 degrees C and pH7. Following a two-hour lag phase, complete biodegradation of 100 mg/l toluene or benzene occurred within 6 to 8 hours. The addition of nitrate, phosphate, or sulfate at various concentrations were found to have significant influence on both toluene and benzene degradation. In addition, results show that the D8 strain has the ability to degrade monochlorophenols, nitrophenols, and phenol, but not aliphatic compounds. Inoculation of groundwater samples containing 100 mg/l toluene or benzene with Pseudmonas sp. D8 resulted in rapid degradation within 24-33 hours.
分离出了具有将甲苯作为唯一碳源加以利用潜力的假单胞菌属D8菌株。在浓度为100毫克/升时，发现该菌株能在30摄氏度和pH值为7的培养基中高效降解甲苯和苯（单独以及混合状态下）。经过两小时的延迟期后，100毫克/升的甲苯或苯在6至8小时内实现了完全生物降解。发现添加不同浓度的硝酸盐、磷酸盐或硫酸盐对甲苯和苯的降解均有显著影响。此外，结果表明D8菌株有能力降解单氯酚、硝基酚和苯酚，但不能降解脂肪族化合物。用假单胞菌属D8接种含有100毫克/升甲苯或苯的地下水样本，能在24至33小时内实现快速降解。
