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本文引用的文献

1
Homing endonucleases: keeping the house in order.归巢内切酶：维持秩序
Nucleic Acids Res. 1997 Sep 1;25(17):3379-88. doi: 10.1093/nar/25.17.3379.
2
The structure of I-Crel, a group I intron-encoded homing endonuclease.I-Crel的结构，一种I类内含子编码的归巢内切酶。
Nat Struct Biol. 1997 Jun;4(6):468-76. doi: 10.1038/nsb0697-468.
3
The galvanization of biology: a growing appreciation for the roles of zinc.生物学的镀锌作用：对锌的作用的认识不断加深。
Science. 1996 Feb 23;271(5252):1081-5. doi: 10.1126/science.271.5252.1081.
4
Substrate binding and turnover by the highly specific I-PpoI endonuclease.高特异性I-PpoI核酸内切酶的底物结合与周转
Biochemistry. 1996 Jan 23;35(3):1076-83. doi: 10.1021/bi952363v.
5
Mechanisms of intron mobility.内含子移动的机制。
J Biol Chem. 1995 Dec 22;270(51):30237-40. doi: 10.1074/jbc.270.51.30237.
6
A family of nuclear homing endonucleases.一个核归巢内切核酸酶家族。
Nucleic Acids Res. 1993 Sep 11;21(18):4405. doi: 10.1093/nar/21.18.4405.
7
Introns as mobile genetic elements.作为可移动遗传元件的内含子。
Annu Rev Biochem. 1993;62:587-622. doi: 10.1146/annurev.bi.62.070193.003103.
8
Interaction of the intron-encoded mobility endonuclease I-PpoI with its target site.内含子编码的可移动内切核酸酶I-PpoI与其靶位点的相互作用。
Mol Cell Biol. 1993 Dec;13(12):7531-9. doi: 10.1128/mcb.13.12.7531-7539.1993.
9
An intron in the nuclear ribosomal DNA of Didymium iridis codes for a group I ribozyme and a novel ribozyme that cooperate in self-splicing.双滴菌核糖体DNA中的一个内含子编码一种I型核酶和一种在自我剪接中协同作用的新型核酶。
Cell. 1994 Feb 25;76(4):725-34. doi: 10.1016/0092-8674(94)90511-8.
10
Protein splicing: self-splicing of genetically mobile elements at the protein level.蛋白质剪接：遗传可移动元件在蛋白质水平上的自我剪接。
Trends Biochem Sci. 1995 Sep;20(9):351-6. doi: 10.1016/s0968-0004(00)89075-1.

多头绒泡菌I-PpoI的结晶及初步X射线研究：一种来自多头绒泡菌的细胞核内、内含子编码的归巢内切酶

Crystallization and preliminary X-ray studies of I-PpoI: a nuclear, intron-encoded homing endonuclease from Physarum polycephalum.

作者信息

Flick K E, McHugh D, Heath J D, Stephens K M, Monnat R J, Stoddard B L

机构信息

Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.

出版信息

Protein Sci. 1997 Dec;6(12):2677-80. doi: 10.1002/pro.5560061226.

DOI:10.1002/pro.5560061226
PMID:9416623
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2143617/
Abstract

The homing endonuclease I-PpoI is encoded by an optional third intron, Pp LSU 3, found in nuclear, extrachromosomal copies of the Physarum polycephalum 26S rRNA gene. This endonuclease promotes the lateral transfer or "homing" of its encoding intron by recognizing and cleaving a partially symmetric, 15 bp homing site in 26S rDNA alleles that lack the Pp LSU 3 intron. The open reading frame encoding I-PpoI has been subcloned, and the endonuclease has been overproduced in E. coli. Purified recombinant I-PpoI has been co-crystallized with a 21 bp homing site DNA duplex. The crystals belong to space group P3(1)21, with unit cell dimensions a = b = 114 A, c = 89 A. The results of initial X-ray diffraction experiments indicate that the asymmetric unit contains an enzyme homodimer and one duplex DNA molecule, and that the unit cell has a specific volume of 3.4 A3/dalton. These experiments also provide strong evidence that I-PpoI contains several bound zinc ions as part of its structure.

摘要

归巢核酸内切酶I-PpoI由一个可选的第三个内含子Pp LSU 3编码，该内含子存在于多头绒泡菌26S rRNA基因的核内、染色体外拷贝中。这种核酸内切酶通过识别并切割26S rDNA等位基因中一个部分对称的15 bp归巢位点来促进其编码内含子的侧向转移或“归巢”，这些等位基因缺乏Pp LSU 3内含子。编码I-PpoI的开放阅读框已被亚克隆，并且该核酸内切酶已在大肠杆菌中过量表达。纯化的重组I-PpoI已与一个21 bp归巢位点DNA双链体共结晶。晶体属于空间群P3(1)21，晶胞参数a = b = 114 Å，c = 89 Å。初步X射线衍射实验结果表明，不对称单元包含一个酶同二聚体和一个双链DNA分子，并且晶胞的比容为3.4 Å3/道尔顿。这些实验还提供了有力证据，表明I-PpoI在其结构中包含几个结合的锌离子。