Koike K, Zhang Z X, Sakamoto Y, Kanda Y, Murakami K, Miyake A, Inoue M
Department of Obstetrics and Gynecology, School of Medicine, Kanazawa University, Ishikawa, Japan.
Life Sci. 1997;61(25):2491-7. doi: 10.1016/s0024-3205(97)00984-3.
Numerous functions have been ascribed to anterior pituitary folliculo-stellate (FS) cells. Recently, we established a pituitary FS-like cell line (TtT/GF) from an isologously transplantable pituitary thyrotropic tumor. Here we investigated the effect of FS cells on prolactin (PRL) secretion by using a co-culture system between TtT/GF cells and GH3 cells. We observed that co-culture with TtT/GF cells significantly increased basal PRL secretion without affecting the growth rate of GH3 cells. Further, addition of anti-cytokine-induced neutrophil chemoattractant (CINC) antibody partially reduced PRL secretion in co-culture of GH3 cells with 10% TtT/GF cells. Co-culture with 5% TtT/GF cells also markedly enhanced the PRL response to thyrotropin-releasing hormone (TRH) by GH3 cells, while addition of anti-CINC antibody had no effects on the enhanced PRL response to TRH. These results suggest that FS cells augment basal PRL secretion and enhance the secretory response of PRL to TRH by a paracrine mechanism.
腺垂体滤泡星状（FS）细胞具有多种功能。最近，我们从可同种异体移植的垂体促甲状腺激素瘤中建立了一种垂体FS样细胞系（TtT/GF）。在此，我们通过使用TtT/GF细胞与GH3细胞的共培养系统，研究了FS细胞对催乳素（PRL）分泌的影响。我们观察到，与TtT/GF细胞共培养显著增加了基础PRL分泌，而不影响GH3细胞的生长速率。此外，添加抗细胞因子诱导的中性粒细胞趋化因子（CINC）抗体可部分降低GH3细胞与10% TtT/GF细胞共培养时的PRL分泌。与5% TtT/GF细胞共培养也显著增强了GH3细胞对促甲状腺激素释放激素（TRH）的PRL反应，而添加抗CINC抗体对增强的PRL对TRH反应没有影响。这些结果表明，FS细胞通过旁分泌机制增加基础PRL分泌并增强PRL对TRH的分泌反应。
