甲状旁腺激素、甲状旁腺激素相关蛋白及降钙素基因相关肽对人胎儿-胎盘循环的血管舒张作用。

Vasodilator effects of parathyroid hormone, parathyroid hormone-related protein, and calcitonin gene-related peptide in the human fetal-placental circulation.

作者信息

Mandsager N T, Brewer A S, Myatt L

机构信息

Department of Obstetrics and Gynecology, University of Cincinnati College of Medicine, OH 45267-0526, USA.

出版信息

J Soc Gynecol Investig. 1994 Jan-Mar;1(1):19-24. doi: 10.1177/107155769400100105.

DOI:10.1177/107155769400100105

PMID:9419741

Abstract

OBJECTIVE

The purpose of our study was to determine the vasoactivity of calcitonin gene-related peptide (CGRP), parathyroid hormone (PTH), and parathyroid hormone-related protein (PTHrP) in the human fetal-placental circulation in vitro.

METHODS

Dually perfused placental cotyledons from term pregnancies were used in this study.

RESULTS

Calcitonin gene-related peptide, PTHrP (both 10(-10)-10(-6) mol/L), and PTH (10(-8)-10(-6) mol/L) demonstrated a significant concentration-dependent vasodilator effect (P = .0007, P = .0172, P = .0063, respectively), following preconstriction with a thromboxane mimetic U46619. The CGRP-1 receptor inhibitor CGRP8-37 (10(-6) mol/L) significantly inhibited (P = .0131) the CGRP-induced vasodilator effect, while the nitric oxide synthesis inhibitor n-nitro-l-arginine showed no inhibitory effect.

CONCLUSIONS

These results demonstrate the vasodilator effects of CGRP, PTH, and PTHrP in the human fetal-placental circulation. Calcitonin gene-related peptide and PTHrP were of equal potency, and both were approximately 100 times more potent than PTH. This study also suggests the CGRP may exert its vasodilator effect through two classes of receptors in the human placenta and may do so independently of nitric oxide.

摘要

目的

我们研究的目的是在体外确定降钙素基因相关肽（CGRP）、甲状旁腺激素（PTH）和甲状旁腺激素相关蛋白（PTHrP）在人胎儿 - 胎盘循环中的血管活性。

方法

本研究使用来自足月妊娠的双灌注胎盘小叶。

结果

在用血栓素类似物U46619预收缩后，降钙素基因相关肽、PTHrP（均为10⁻¹⁰ - 10⁻⁶mol/L）和PTH（10⁻⁸ - 10⁻⁶mol/L）均表现出显著的浓度依赖性血管舒张作用（分别为P = 0.0007，P = 0.0172，P = 0.0063）。CGRP - 1受体抑制剂CGRP8 - 37（10⁻⁶mol/L）显著抑制（P = 0.0131）CGRP诱导的血管舒张作用，而一氧化氮合成抑制剂L-硝基精氨酸未显示出抑制作用。