利用消毒剂松油筛选大肠杆菌多重耐药（mar）突变体：mar和acrAB基因座的作用
Selection of multiple-antibiotic-resistant (mar) mutants of Escherichia coli by using the disinfectant pine oil: roles of the mar and acrAB loci.
作者信息
Moken M C, McMurry L M, Levy S B
出版信息
Antimicrob Agents Chemother. 1997 Dec;41(12):2770-2. doi: 10.1128/AAC.41.12.2770.
Abstract
Mutants of Escherichia coli selected for resistance to the disinfectant pine oil or to a household product containing pine oil also showed resistance to multiple antibiotics (tetracycline, ampicillin, chloramphenicol, and nalidixic acid) and overexpressed the marA gene. Likewise, antibiotic-selected Mar mutants, which also overexpress marA, were resistant to pine oil. Deletion of the mar or acrAB locus, the latter encoding a multidrug efflux pump positively regulated in part by MarA, increased the susceptibility of wild-type and mutant strains to pine oil.
摘要
经筛选获得的对消毒剂松油或含松油的家用产品具有抗性的大肠杆菌突变体，也对多种抗生素（四环素、氨苄青霉素、氯霉素和萘啶酸）具有抗性，并且marA基因过度表达。同样，经抗生素筛选的Mar突变体（其marA基因也过度表达）对松油具有抗性。mar或acrAB基因座的缺失（后者编码一种部分受MarA正向调控的多药外排泵）增加了野生型和突变株对松油的敏感性。
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