Erjefält J S, Korsgren M, Nilsson M C, Sundler F, Persson C G
Department of Physiology and Neuroscience, University of Lund, Sweden.
Clin Exp Allergy. 1997 Nov;27(11):1344-55.
Associations between allergen challenge-induced sites of epithelial damage and the distribution of leucocytes and extravasated plasma remain unexplored.
To study neutrophils, eosinophils, and fibrinogen at allergen challenge-induced patchy epithelial damage-restitution sites in guinea-pig trachea.
After local challenge tracheal tissue (cryo sections and whole-mounts) and lumen (selective tracheal lavage) were examined at 1, 5, and 24 h. Eosinophils, neutrophils and fibrinogen were identified by histochemistry.
Neutrophils increased markedly in tracheal lavage fluids and in tissue and were strongly associated with the challenge-induced epithelial craters of damage-restitution. At 1 and 24 h eosinophils were increased in the tracheal lumen whereas the surrounding tissue displayed a reversed pattern. Gels rich in fibrinogen, neutrophils, and eosinophils were present in epithelial crater areas, protruding into the lumen. Clusters of free eosinophil granules, Cfegs, released through lysis of eosinophils, and neutrophils with long cytoplasmatic protrusions abounded in these crater areas.
The present findings provide important new insights into allergic airways where sites of epithelial damage-restitution processes emerge as the major loci for eosinophil, neutrophil, and plasma protein activities, the latter likely causing leukocyte adhesion and activation in vivo. The distribution of eosinophils in this study suggests roles of these cells both in airway mucosa and in regional lymph nodes. Based on the present study we also propose that lysis of eosinophils and Cfegs generation are a major paradigm for activation of these cells in vivo.
变应原激发诱导的上皮损伤部位与白细胞和渗出血浆分布之间的关联尚未得到探索。
研究豚鼠气管变应原激发诱导的斑片状上皮损伤修复部位的中性粒细胞、嗜酸性粒细胞和纤维蛋白原。
局部激发后，在1、5和24小时检查气管组织（冰冻切片和整装片）和管腔（选择性气管灌洗）。通过组织化学鉴定嗜酸性粒细胞、中性粒细胞和纤维蛋白原。
气管灌洗液和组织中的中性粒细胞显著增加，且与激发诱导的损伤修复上皮凹陷密切相关。在1小时和24小时时，气管管腔内嗜酸性粒细胞增加，而周围组织呈现相反的模式。富含纤维蛋白原、中性粒细胞和嗜酸性粒细胞的凝胶存在于上皮凹陷区域，并突出到管腔中。这些凹陷区域有大量通过嗜酸性粒细胞溶解释放的游离嗜酸性粒细胞颗粒簇（Cfegs）以及具有长细胞质突起的中性粒细胞。
本研究结果为过敏性气道提供了重要的新见解，其中上皮损伤修复过程的部位成为嗜酸性粒细胞、中性粒细胞和血浆蛋白活动的主要场所，后者可能在体内导致白细胞黏附和激活。本研究中嗜酸性粒细胞的分布表明这些细胞在气道黏膜和区域淋巴结中均发挥作用。基于本研究，我们还提出嗜酸性粒细胞的溶解和Cfegs的产生是这些细胞在体内激活的主要模式。
