Stoyanov A V, Righetti P G
Institute of Gene Biology, Russian Academy of Sciences, Moscow.
Electrophoresis. 1997 Oct;18(11):1944-50. doi: 10.1002/elps.1150181111.
Two different approaches (stepwise and parallel mechanism) for describing the dissociation of amphoteric protolytes are described. Additionally, the classification of the possible model on the basis of the ampholyte lifetime state is proposed. The possibility of application of different schemes is discussed. It is suggested that the correct description may be based only on highly relaxing models. The formulas describing the properties of ampholyte solutions are derived for the case of two ionogenic groups. It is demonstrated that the incorrect choice of the dissociation model may result in essential mistakes in calculation of such values as buffer capacity, electrophoretic mobility, and conductivity. On the basis of the theory here developed, the interpretation of natural pH-gradient conductivity is given. Some terminology questions are also discussed.
描述了两种用于描述两性电解质解离的不同方法（逐步机制和平行机制）。此外，还提出了基于两性电解质寿命状态的可能模型分类。讨论了不同方案的应用可能性。建议正确的描述可能仅基于高度松弛的模型。推导了描述具有两个离子ogenic基团情况下两性电解质溶液性质的公式。结果表明，解离模型选择不当可能导致在计算诸如缓冲容量、电泳迁移率和电导率等数值时出现重大错误。基于此处发展的理论，给出了对自然pH梯度电导率的解释。还讨论了一些术语问题。
