一种由昆虫逆转录病毒吉普赛包膜假型化的基于莫洛尼鼠白血病病毒的逆转录病毒载体可感染果蝇细胞。
A Moloney murine leukemia virus-based retroviral vector pseudotyped by the insect retroviral gypsy envelope can infect Drosophila cells.
作者信息
Teysset L, Burns J C, Shike H, Sullivan B L, Bucheton A, Terzian C
机构信息
Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France.
出版信息
J Virol. 1998 Jan;72(1):853-6. doi: 10.1128/JVI.72.1.853-856.1998.
Abstract
The gypsy element of Drosophila melanogaster is the first retrovirus identified so far in invertebrates. Previous data suggest that gypsy ENV-like ORF3 mediates viral infectivity. We have produced in the 293GP/LNhsp701ucL.3 human cell line a Moloney murine leukemia virus-based retroviral vector pseudotyped by the gypsy ENV-like protein. We have shown by immunostaining that the gypsy envelope protein is produced in 293GP/LNhsp701ucL.3 cells and that vector particles collected from these cells can infect Drosophila cells. Our results provide direct evidence that the infectious property of gypsy is due to its ORF3 gene product.
摘要
果蝇的吉普赛元件是迄今为止在无脊椎动物中鉴定出的首个逆转录病毒。先前的数据表明，吉普赛ENV样ORF3介导病毒感染性。我们在293GP/LNhsp701ucL.3人细胞系中构建了一种基于莫洛尼鼠白血病病毒的逆转录病毒载体，该载体由吉普赛ENV样蛋白进行假型化。我们通过免疫染色表明，吉普赛包膜蛋白在293GP/LNhsp701ucL.3细胞中产生，并且从这些细胞收集的载体颗粒可以感染果蝇细胞。我们的结果提供了直接证据，证明吉普赛的感染特性归因于其ORF3基因产物。
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