Haro H, Komori H, Okawa A, Murakami S, Muneta T, Shinomiya K
Department of Orthopaedic Surgery, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
J Orthop Res. 1997 Sep;15(5):734-41. doi: 10.1002/jor.1100150516.
We previously demonstrated that the granulation tissues of herniated nucleus pulposus are composed of a marked infiltration of macrophages that strongly express monocyte chemotactic protein-1. Monocyte chemotactic protein-1 is a chemotactic cytokine that contributes to the activation and recruitment of macrophages. Relatively little is known about its role in the resorption process of herniated nucleus pulposus. To clarify the sequential dynamics of expression of monocyte chemotactic protein-1 in the granulation tissues of herniated nucleus pulposus, we introduced a rat autologous transplantation model of nuclear materials onto its lumbar dura mater and performed immunohistological analysis and competitive polymerase chain reaction assay using the grafted samples. Immunohistological analysis demonstrated that the majority of infiltrating mononuclear cells expressed monocyte chemotactic protein-1. Monocyte chemotactic protein-1 mRNA was expressed in the first 3 weeks after the procedure and was significantly and maximally upregulated at 1 week. To determine whether human recombinant monocyte chemotactic protein-1 facilitates the resorption process of herniated nucleus pulposus, we introduced another model of autologous transplantation, wherein the nuclear materials were grafted to the abdominal subcutaneous tissues and recombinant monocyte chemotactic protein-1 was subsequently applied to these materials. When monocyte chemotactic protein-1 was injected into the murine nucleus pulposus tissues, they reduced in size more rapidly than in the control group. These findings suggest that monocyte chemotactic protein-1 plays an important role in the recruitment of macrophages in the early phase of the resorption process of herniated nucleus pulposus and that its application may physiologically facilitate the resorption process of the nucleus pulposus.
我们之前证明，突出的髓核的肉芽组织由大量强烈表达单核细胞趋化蛋白-1的巨噬细胞浸润组成。单核细胞趋化蛋白-1是一种趋化细胞因子，有助于巨噬细胞的激活和募集。关于其在突出髓核吸收过程中的作用，人们了解相对较少。为了阐明单核细胞趋化蛋白-1在突出髓核肉芽组织中表达的连续动态变化，我们将大鼠自体核物质移植模型应用于其腰段硬脑膜，并使用移植样本进行免疫组织学分析和竞争性聚合酶链反应检测。免疫组织学分析表明，大多数浸润的单核细胞表达单核细胞趋化蛋白-1。单核细胞趋化蛋白-1 mRNA在手术后的前3周表达，并在第1周显著上调且达到最大值。为了确定重组人单核细胞趋化蛋白-1是否促进突出髓核的吸收过程，我们引入了另一种自体移植模型，即将核物质移植到腹部皮下组织，随后将重组单核细胞趋化蛋白-1应用于这些物质。当将单核细胞趋化蛋白-1注入小鼠髓核组织时，它们的尺寸比对照组更快减小。这些发现表明，单核细胞趋化蛋白-1在突出髓核吸收过程的早期巨噬细胞募集中起重要作用，并且其应用可能在生理上促进髓核的吸收过程。
