原纤蛋白-1在人类足月胎盘中的定位。
Localization of fibrillin-1 in the human term placenta.
作者信息
Jacobson S L, Kimberly D, Thornburg K, Maslen C
机构信息
Department of Obstetrics and Gynecology, Oregon Health Sciences University, Portland 97201, USA.
出版信息
J Soc Gynecol Investig. 1995 Sep-Oct;2(5):686-90. doi: 10.1016/1071-5576(95)97405-6.
OBJECTIVE
We determined the level of message for fibrillin-1, a single-stranded glycoprotein found in the elastic microfibrils of the extracellular matrix, in human placenta compared with other adult human tissues and localized the protein in the term human placenta.
METHODS
Northern blot analysis using a cDNA specific for fibrillin-1 was used to compare message levels in several adult human tissues and placenta. Immunohistochemical staining, using a monoclonal antibody to human fibrillin-1, was used to visualize the site of fibrillin-1 in term human placenta.
RESULTS
Larger amounts of message for fibrillin-1 were present in placenta than in any of the other tissues tested. Staining for fibrillin-1 was present in the villous stroma but not in the basement membranes, trophoblast cells, or in blood vessels of stem, mature intermediate, or terminal villi.
CONCLUSIONS
Fibrillin-1 is distributed extensively in the villous stroma of the term human placenta and may contribute elastic properties to the placenta and basal plate required for an active uterus.
目的
我们测定了原纤蛋白-1（一种存在于细胞外基质弹性微原纤维中的单链糖蛋白）在人胎盘中相对于其他成人组织的信使水平，并在足月人胎盘中定位了该蛋白。
方法
使用针对原纤蛋白-1的cDNA进行Northern印迹分析，以比较几种成人组织和胎盘中的信使水平。使用抗人原纤蛋白-1单克隆抗体进行免疫组织化学染色，以观察足月人胎盘中原纤蛋白-1的位点。
结果
胎盘中存在的原纤蛋白-1信使量比所测试的任何其他组织都多。原纤蛋白-1染色存在于绒毛基质中，但不存在于基底膜、滋养层细胞或干绒毛、成熟中间绒毛或终末绒毛的血管中。
结论
原纤蛋白-1广泛分布于足月人胎盘的绒毛基质中，并可能为活跃子宫所需的胎盘和基底板贡献弹性特性。
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