Budde U, Drewke E
Allgemeines Krankenhaus Harburg, Hamburg, Germany.
Beitr Infusionsther Transfusionsmed. 1994;32:408-14.
The von Willebrand factor (vWf) stabilizes F VIII in plasma and is involved in platelet adhesion at high shear rates. While the first function is independent of the grade of multimerization, the latter needs the full range of large vWf multimers. Recently new virus-inactivated plasmas and F VIII concentrates, double-inactivated by heat and chemical treatment, became available. To evaluate whether these treatments have detrimental effects on the structure of the vWf protein we compared some of these products with fresh frozen plasma (FFP), and the Biotest F VIII concentrate with two F VIII concentrates inactivated by pasteurization or solvent/detergent (SD) treatment. The large vWf multimeres were preserved in single-donor plasmas and laboratory plasma pools. All commercial pool products showed a loss of the largest vWf multimers. While SD-treated plasma and the F VIII concentrates from Behring and Intersero contained enough large vWf multimers to allow treatment of patients with von Willebrand disease (vWd), the Biotest F VIII concentrate showed a loss of large and some intermediate multimers and is less suitable for the treatment of patients with vWd.
血管性血友病因子（vWf）可稳定血浆中的F VIII，并在高剪切速率下参与血小板黏附。虽然第一个功能与多聚体的级别无关，但后者需要完整范围的大vWf多聚体。最近，出现了经热和化学处理双重灭活的新型病毒灭活血浆和F VIII浓缩物。为评估这些处理对vWf蛋白结构是否有不利影响，我们将其中一些产品与新鲜冰冻血浆（FFP）进行了比较，并将百泰F VIII浓缩物与两种经巴氏消毒或溶剂/去污剂（SD）处理灭活的F VIII浓缩物进行了比较。大vWf多聚体在单供体血浆和实验室血浆库中得以保留。所有商业混合产品均显示最大的vWf多聚体有所损失。虽然经SD处理的血浆以及拜耳和英特龙的F VIII浓缩物含有足够的大vWf多聚体，可用于治疗血管性血友病（vWd）患者，但百泰F VIII浓缩物显示大的和一些中间多聚体有所损失，不太适合用于治疗vWd患者。
