O'Neill A J, Cotter T G, Russell J M, Gaffney E F
Department of Histopathology, St James's Hospital, Dublin, Ireland.
J Pathol. 1997 Nov;183(3):325-9. doi: 10.1002/(SICI)1096-9896(199711)183:3<325::AID-PATH941>3.0.CO;2-A.
Abl kinases encoded by the abl oncogenes inhibit apoptosis without affecting cell proliferation. The aim of this study was to examine a wide range of normal fetal and adult human tissues and a variety of tumour types for Abl immunoreactivity. Sections from 193 paraffin blocks of normal fetal and adult tissues and 72 blocks from representative tumours were stained immunohistochemically using a polyclonal antibody to c-Abl/Bcr-Abl oncoprotein. Weak Abl immunoreactivity was observed in many adult tissues. Moderately intense or strong staining (cytoplasmic, nuclear or membranous) was consistently seen in hyaline cartilage, adipocytes, and ciliated epithelium. In fetal tissues, there was a broadly similar staining pattern, but Abl expression was also seen in muscle (all types) and occasionally in endothelial cells. The most intense staining was seen in sites of endochondral ossification and in the umbilical cord stroma. Negatively staining tissues included epidermis and squamous mucosa, lymph nodes, tonsil, spleen, hepatocytes, and adrenals. Most tumours showed focal or weak Abl immunoreactivity. The most intense staining was seen in chondrosarcoma, liposarcoma, and diffuse gastric (signet ring) adenocarcinoma. In the latter two tumour types, Abl expression was also observed in tumour microvessels. These results suggest that Abl not only functions as an apoptosis inhibitor, but also may have a role in connective tissue maturation and differentiation and in tumour growth and angiogenesis.
由abl癌基因编码的Abl激酶可抑制细胞凋亡而不影响细胞增殖。本研究旨在检测多种正常胎儿和成人组织以及多种肿瘤类型中的Abl免疫反应性。使用针对c-Abl/Bcr-Abl癌蛋白的多克隆抗体，对193个正常胎儿和成人组织石蜡块切片以及72个代表性肿瘤组织块进行免疫组织化学染色。在许多成人组织中观察到较弱的Abl免疫反应性。在透明软骨、脂肪细胞和纤毛上皮中始终可见中度强烈或强烈染色（细胞质、细胞核或细胞膜）。在胎儿组织中，染色模式大致相似，但在肌肉（所有类型）中也可见Abl表达，偶尔在内皮细胞中也有表达。在软骨内成骨部位和脐带基质中可见最强的染色。阴性染色组织包括表皮和鳞状黏膜、淋巴结、扁桃体、脾脏、肝细胞和肾上腺。大多数肿瘤显示局灶性或较弱的Abl免疫反应性。在软骨肉瘤、脂肪肉瘤和弥漫性胃（印戒）腺癌中可见最强的染色。在后两种肿瘤类型中，在肿瘤微血管中也观察到Abl表达。这些结果表明，Abl不仅作为一种凋亡抑制剂发挥作用，而且可能在结缔组织成熟和分化以及肿瘤生长和血管生成中起作用。
