乙肝抗原吸附到胶体二氧化硅后洗脱的最佳条件。
Optimal conditions for elution of hepatitis B antigen after absorption onto colloidal silica.
作者信息
Pillot J, Goueffon S, Keros R G
出版信息
J Clin Microbiol. 1976 Sep;4(3):205-7. doi: 10.1128/jcm.4.3.205-207.1976.
Abstract
Hepatitis B surface antigen (HBSAg) adsorbed from sera onto colloidal silica could be completely eluted through the use of 0.25% sodium deoxycholate in 0.01 M borax, pH 9.3, at 56 degrees C. The HBSAg recovered in the eluate represented 100% of that present in the original serum, and it was contaminated by only trace amounts of serum proteins (in decreasing amounts: beta-lipoprotein, immunoglobulin G, albumin). This preliminary step greatly facilitates purification of large amounts of HBSAg and provides small volumes of highly concentrated material for subsequent purification by density gradient centrifugation.
摘要
从血清吸附到胶体二氧化硅上的乙肝表面抗原（HBSAg），在56℃下，通过使用0.01M硼砂（pH 9.3）中的0.25%脱氧胆酸钠可被完全洗脱。洗脱液中回收的HBSAg占原始血清中HBSAg的100%，且仅被痕量血清蛋白污染（含量递减：β-脂蛋白、免疫球蛋白G、白蛋白）。这一初步步骤极大地促进了大量HBSAg的纯化，并为随后通过密度梯度离心进行纯化提供了少量高浓缩材料。
相似文献
1
Optimal conditions for elution of hepatitis B antigen after absorption onto colloidal silica.乙肝抗原吸附到胶体二氧化硅后洗脱的最佳条件。
J Clin Microbiol. 1976 Sep;4(3):205-7. doi: 10.1128/jcm.4.3.205-207.1976.
2
Immunochemical structure of the hepatitis B surface antigen vaccine--I. Treatment of immobilized HBsAg by dissociation agents with or without enzymatic digestion and identification of polypeptides by protein blotting.乙肝表面抗原疫苗的免疫化学结构——I. 用解离剂处理固定化乙肝表面抗原，有无酶消化及通过蛋白质印迹法鉴定多肽
Mol Immunol. 1984 Jan;21(1):53-60. doi: 10.1016/0161-5890(84)90089-0.
3
Antigenic and physicochemical characterization of Hepatitis B surface protein under extreme temperature and pH conditions.极端温度和 pH 条件下乙型肝炎表面蛋白的抗原性和物理化学特性。
Vaccine. 2019 Oct 8;37(43):6415-6425. doi: 10.1016/j.vaccine.2019.09.005. Epub 2019 Sep 10.
4
Purification of thyroid lysosomes by colloidal silica density gradient centrifugation.通过硅胶密度梯度离心法纯化甲状腺溶酶体。
Endocrinology. 1985 Aug;117(2):580-90. doi: 10.1210/endo-117-2-580.
5
Transport of colloidal silica in unsaturated sand: Effect of charging properties of sand and silica particles.胶体二氧化硅在非饱和砂中的运移：砂和二氧化硅颗粒带电特性的影响。
Chemosphere. 2016 Jul;154:179-186. doi: 10.1016/j.chemosphere.2016.03.105. Epub 2016 Apr 2.
6
A method fof production or antisera to hepatitis b antigen subtypes, and the distribution of subtypes d and y among norwegian hepatitis b patients.一种生产抗乙型肝炎抗原亚型血清的方法，以及挪威乙型肝炎患者中d和y亚型的分布情况。 （注：原文中“fof”应改为“for”）
Acta Pathol Microbiol Scand B. 1975 Apr;83(2):153-6. doi: 10.1111/j.1699-0463.1975.tb00086.x.
7
Large scale purification of hepatitis B surface antigen (HBsAg).乙型肝炎表面抗原（HBsAg）的大规模纯化。
Ann Microbiol (Paris). 1978 Jul;129B(1):87-100.
8
Large-scale purification of hepatitis B surface antigen.乙型肝炎表面抗原的大规模纯化。
J Clin Microbiol. 1976 Jun;3(6):626-31. doi: 10.1128/jcm.3.6.626-631.1976.
9
Rapid purification of lymphocytic choriomeningitis virus by density gradient centrifugation in colloidal silica.通过在胶体二氧化硅中进行密度梯度离心快速纯化淋巴细胞性脉络丛脑膜炎病毒。
J Gen Virol. 1979 Jan;42(1):215-8. doi: 10.1099/0022-1317-42-1-215.
10
Purification and application of a single-chain Fv antibody fragment specific to hepatitis B virus surface antigen.乙肝病毒表面抗原特异性单链Fv抗体片段的纯化及应用
Biotechniques. 1995 Oct;19(4):606-8, 610, 612 passim.
引用本文的文献
1
A food-grade process for isolation and partial purification of bacteriocins of lactic acid bacteria that uses diatomite calcium silicate.一种使用硅藻土硅酸钙从乳酸菌中分离和部分纯化细菌素的食品级工艺。
Appl Environ Microbiol. 1996 May;62(5):1764-9. doi: 10.1128/aem.62.5.1764-1769.1996.
2
Multiple chemical forms of hepatitis B surface antigen produced in yeast.酵母中产生的多种化学形式的乙型肝炎表面抗原。
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6830-4. doi: 10.1073/pnas.82.20.6830.
本文引用的文献
1
The purification of hepatitis-associated antigen (HAA-SH-AU) from human serum and the preparation of a sheep anti-HAA serum.从人血清中纯化肝炎相关抗原（HAA-SH-AU）及制备羊抗-HAA血清。
Vox Sang. 1972;23(4):249-55. doi: 10.1111/j.1423-0410.1972.tb03458.x.
2
On the adsorption of Australia antigen to a colloidal silica, and some possible applications.关于澳大利亚抗原在胶体二氧化硅上的吸附及一些可能的应用。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(6):935-6. doi: 10.1111/j.0365-5563.1973.tb00025.x.
3
Dissociation of antibody from erythrocyte surfaces by chaotropic ions.离液离子使抗体从红细胞表面解离。
J Immunol. 1971 Mar;106(3):673-80.
4
Ultracentrifuge studies on the effects of thiocyanate ion on antigen--antibody systems.关于硫氰酸根离子对抗抗原-抗体系统影响的超速离心研究。
Immunochemistry. 1969 Jan;6(1):77-83. doi: 10.1016/0019-2791(69)90180-3.
5
Concentration and purification of hepatitis B antigen with polyethylene glycol and polyelectrolyte 60, a cross-linked copolymer of isobutylene maleic anhydride.用聚乙二醇和聚电解质60（异丁烯马来酸酐的交联共聚物）对乙肝抗原进行浓缩和纯化。
Infect Immun. 1972 Sep;6(3):335-8. doi: 10.1128/iai.6.3.335-338.1972.
6
Viral hepatitis type B: propects for active immunization.乙型病毒性肝炎：主动免疫的前景
Dev Biol Stand. 1975;30:363-7.
7
The problem of purification of HBs antigen.乙肝表面抗原的纯化问题。
Dev Biol Stand. 1975;30:11-17.
8
Protein chromatography on adsorbents with hydrophobic and ionic groups. Some properties of N-(3-carboxypropionyl)aminodecyl-sepharose and its interaction with wheat-germ aspartate transcarbamoylase.基于具有疏水基团和离子基团吸附剂的蛋白质色谱法。N-(3-羧基丙酰基)氨基癸基琼脂糖凝胶的一些性质及其与小麦胚芽天冬氨酸转氨甲酰酶的相互作用
Biochem J. 1975 Nov;151(2):281-90. doi: 10.1042/bj1510281.
Zotero 插件