Pillot J, Goueffon S, Keros R G
J Clin Microbiol. 1976 Sep;4(3):205-7. doi: 10.1128/jcm.4.3.205-207.1976.
Hepatitis B surface antigen (HBSAg) adsorbed from sera onto colloidal silica could be completely eluted through the use of 0.25% sodium deoxycholate in 0.01 M borax, pH 9.3, at 56 degrees C. The HBSAg recovered in the eluate represented 100% of that present in the original serum, and it was contaminated by only trace amounts of serum proteins (in decreasing amounts: beta-lipoprotein, immunoglobulin G, albumin). This preliminary step greatly facilitates purification of large amounts of HBSAg and provides small volumes of highly concentrated material for subsequent purification by density gradient centrifugation.
从血清吸附到胶体二氧化硅上的乙肝表面抗原(HBSAg),在56℃下,通过使用0.01M硼砂(pH 9.3)中的0.25%脱氧胆酸钠可被完全洗脱。洗脱液中回收的HBSAg占原始血清中HBSAg的100%,且仅被痕量血清蛋白污染(含量递减:β-脂蛋白、免疫球蛋白G、白蛋白)。这一初步步骤极大地促进了大量HBSAg的纯化,并为随后通过密度梯度离心进行纯化提供了少量高浓缩材料。