乙型肝炎表面抗原的大规模纯化。
Large-scale purification of hepatitis B surface antigen.
作者信息
Vnek J, Prince A M
出版信息
J Clin Microbiol. 1976 Jun;3(6):626-31. doi: 10.1128/jcm.3.6.626-631.1976.
Abstract
Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02 M sodium phosphate buffer.
摘要
通过两个简单的纯化步骤从血浆中浓缩和纯化乙型肝炎表面抗原。第一步，通过聚乙二醇沉淀将抗原纯化24倍。通过分批吸附到羟基磷灰石上并随后用0.02M磷酸钠缓冲液洗脱实现了额外的10倍纯化。
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