肝脏提取物对磷酸烯醇式丙酮酸羧激酶（GTP）的失活作用。
Inactivation of phosphoenolypyruvate carboxykinase (GTP) by liver extracts.
作者信息
Ballard F J, Hopgood M F
出版信息
Biochem J. 1976 Mar 15;154(3):717-24. doi: 10.1042/bj1540717.
Abstract
- The inactivation of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) in liver extracts was catalysed by the microsomal fraction, and led to the enzyme becoming bound to the microsomal membranes. 2. Inactivation by microsomal fraction, typsin or heating at 48degreesC was accelerated by L-cystine, D-cystine and oxidized glutathione and decreased by dithiothreitol. 3. MnC1(2) and CoC1(2) protected the enzyme from inactivation by heat or microsomal fraction, but did not affect the inactivation caused by trypsin. 4. Several proteinase inhibitors had no effect on the microsomal inactivation reaction, suggesting that proteolysis was not involved. 5. It is argued that the initial step in the degradation of phosphoenolpyruvate carboxykinase (GTP) is an inactivation reaction, perhaps involving oxidized thiol compounds.
摘要
- 微粒体部分催化肝脏提取物中磷酸烯醇丙酮酸羧激酶（GTP）（EC 4.1.1.32）的失活，并导致该酶与微粒体膜结合。2. L-胱氨酸、D-胱氨酸和氧化型谷胱甘肽可加速微粒体部分、胰蛋白酶或48℃加热导致的失活，而二硫苏糖醇可使其失活作用减弱。3. MnC1₂和CoC1₂可保护该酶免受热或微粒体部分导致的失活影响，但不影响胰蛋白酶引起的失活。4. 几种蛋白酶抑制剂对微粒体失活反应无影响，这表明该反应不涉及蛋白质水解。5. 有人认为，磷酸烯醇丙酮酸羧激酶（GTP）降解的第一步是失活反应，可能涉及氧化型硫醇化合物。
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2
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