半胱氨酸和胱氨酸在大鼠肝脏匀浆中酪氨酸转氨酶失活过程中的作用。
Participation of cysteine and cystine in inactivation of tyrosine aminotransferase in rat liver homogenates.
作者信息
Buckley W T, Milligan L P
出版信息
Biochem J. 1978 Nov 15;176(2):449-54. doi: 10.1042/bj1760449.
Abstract
- Inactivation of tyrosine aminotransferase was studied in rat liver homogenates. Under an O2 atmosphere with cysteine added, inactivation was rapid after a lag period of approx. 1h, whereas a N2 atmosphere extended the lag period to approx. 3h. 2. Replacement of cysteine with cystine resulted in rapid inactivation both aerobically and anaerobically. 3. Removal of the particulate fraction by centrifuging rat liver homogenates at 13,000g for 9min resulted in an aerobic lag period of 0.5h in the presence of cystine and approx. 3h in the presence of cysteine. 4. It is proposed that the stimulatory effect of cysteine on tyrosine aminotransferase inactivation occurs largely as a result of oxidation to cystine, which appears to be a more directly effective agent.
摘要
- 在大鼠肝脏匀浆中研究了酪氨酸转氨酶的失活情况。在添加半胱氨酸的氧气氛围下，经过约1小时的延迟期后失活迅速，而在氮气氛围下，延迟期延长至约3小时。2. 用胱氨酸替代半胱氨酸导致在需氧和厌氧条件下均迅速失活。3. 通过在13,000g下离心大鼠肝脏匀浆9分钟去除颗粒部分，在存在胱氨酸的情况下需氧延迟期为0.5小时，在存在半胱氨酸的情况下约为3小时。4. 有人提出，半胱氨酸对酪氨酸转氨酶失活的刺激作用主要是由于氧化为胱氨酸的结果，胱氨酸似乎是一种更直接有效的试剂。
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