Tiflova O A, Leonov P G, Karbysheva E A, Shakhnabatian L G
Mikrobiologiia. 1997 Sep-Oct;66(5):640-3.
The influence of the of radiation a He-Ne laser (632.8 nm, 30 W/m2, 5-20 J/m2) on the transformation of Escherichia coli cells with plasmid DNA was studied. The irradiation of a mixture of bacterial cells and plasmid DNA increased the transformation efficiency 2.5-3 times, thus offering an alternative to the heat treatment commonly used. In contrast to the standard techniques, the laser-induced increase in the transformation efficiency was accompanied by a 1.7- to 2-fold increase in cell survival. The effect of the 632.8-nm light, know to be absorbed by membrane porphyrin components, is supposed to be mediated via a modification in the replication and transformation DNA-membrane complexes in E. coli cells.
研究了氦氖激光（632.8纳米，30瓦/平方米，5 - 20焦耳/平方米）辐射对用质粒DNA转化大肠杆菌细胞的影响。对细菌细胞和质粒DNA混合物进行照射可使转化效率提高2.5至3倍，从而提供了一种常用热处理方法的替代方案。与标准技术不同，激光诱导的转化效率提高伴随着细胞存活率提高1.7至2倍。已知632.8纳米的光可被膜卟啉成分吸收，其效应被认为是通过对大肠杆菌细胞中复制和转化DNA - 膜复合物的修饰来介导的。
