果蝇中19 kDa信号识别颗粒蛋白基因座的同源物。

A homologue of the 19 kDa signal recognition particle protein locus in Drosophila melanogaster.

作者信息

Lai C, Langley C H

机构信息

Center for Population Biology, University of California, Davis 95616, USA.

出版信息

Gene. 1997 Dec 5;203(1):59-63. doi: 10.1016/s0378-1119(97)00492-7.

DOI:10.1016/s0378-1119(97)00492-7

PMID:9426007

Abstract

A homologue of 19 kDa signal recognition particle locus (SRP19) was cloned and molecularly characterized in Drosophila melanogaster. It is located in the 65F region of the left arm on the third chromosome, approx. 500 bp 5' of the quemao locus. The SRP19 transcript was determined from cDNA clones, Northern blot analysis, and the 5' rapid amplification of cDNA end method. SRP19 was expressed in all the developmental stages of Drosophila. The predicted amino acid sequence (163 aa) shows that SRP19 of Drosophila shares 44%, 29%, 17% and 19% identity with the homologues from human, rice and two yeast species (Saccharomyces and Yarrowia), respectively. The most conserved amino acid residues across these species are located at those sites required for in vitro association with the 7S RNA component of the SRP.

摘要

在黑腹果蝇中克隆并对19 kDa信号识别颗粒基因座（SRP19）的一个同源物进行了分子特征分析。它位于第三条染色体左臂的65F区域，在quemao基因座的5'端约500 bp处。通过cDNA克隆、Northern印迹分析和cDNA末端快速扩增方法确定了SRP19转录本。SRP19在果蝇的所有发育阶段均有表达。预测的氨基酸序列（163个氨基酸）表明，果蝇的SRP19与人、水稻以及两种酵母物种（酿酒酵母和解脂耶氏酵母）的同源物分别具有44%、29%、17%和19%的同一性。这些物种中最保守的氨基酸残基位于体外与SRP的7S RNA组分结合所需的位点。