Katis V L, Harry E J, Wake R G
Department of Biochemistry, University of Sydney, NSW, Australia.
Mol Microbiol. 1997 Dec;26(5):1047-55. doi: 10.1046/j.1365-2958.1997.6422012.x.
The Bacillus subtilis divIC gene is involved in the initiation of cell division. It encodes a 14.7 kDa protein, with a potential transmembrane region near the N-terminus. In this paper, we show that DivIC is associated with the cell membrane and, in conjunction with previously published sequence data, conclude that it is oriented such that its small N-terminus is within the cytoplasm and its larger C-terminus is external to the cytoplasm. DivIC is shown to be a highly abundant division protein, present at approximately 50000 molecules per cell. Using immunofluorescence microscopy, DivIC was seen to localize at the division site of rapidly dividing cells between well-segregated nucleoids. Various DivIC immunostaining patterns were observed, and these correlated with different cell lengths, suggesting that the DivIC localization takes on various forms during the cell cycle. The DivIC immunolocalization patterns are very similar to those of another membrane-bound B. subtilis division protein, DivIB.
枯草芽孢杆菌的divIC基因参与细胞分裂的起始。它编码一种14.7 kDa的蛋白质，在N端附近有一个潜在的跨膜区域。在本文中，我们表明DivIC与细胞膜相关，并且结合先前发表的序列数据，得出结论：它的取向是其较小的N端在细胞质内，而较大的C端在细胞质外。DivIC被证明是一种高度丰富的分裂蛋白，每个细胞中约有50000个分子。使用免疫荧光显微镜观察到，DivIC定位于快速分裂细胞中位于良好分离的类核之间的分裂位点。观察到了各种DivIC免疫染色模式，并且这些模式与不同的细胞长度相关，这表明DivIC在细胞周期中呈现出各种形式的定位。DivIC的免疫定位模式与另一种膜结合的枯草芽孢杆菌分裂蛋白DivIB的模式非常相似。
