通过简单化学处理在钛上快速沉淀磷酸钙层。

Fast precipitation of calcium phosphate layers on titanium induced by simple chemical treatments.

作者信息

Wen H B, Wolke J G, de Wijn J R, Liu Q, Cui F Z, de Groot K

机构信息

Biomaterials Research Group, Leiden University, Bilthoven, The Netherlands.

出版信息

Biomaterials. 1997 Nov;18(22):1471-8. doi: 10.1016/s0142-9612(97)82297-1.

DOI:10.1016/s0142-9612(97)82297-1

PMID:9426176

Abstract

A simple two-step chemical treatment, i.e. etching with HCl and H2SO4 followed by immersion in boiling dilute NaOH solution, has been developed by our group to prepare bioactive microporous titanium surfaces allowing fast deposition of a calcium phosphate layer (CPL) from an in vitro supersaturated calcification solution (SCS). In this work, a precalcification (Pre-Ca) procedure was applied by soaking the two-step treated titanium in Na2HPO4 and then saturated Ca(OH)2 solution before immersion in SCS to accelerate further the CPL precipitation. The treated titanium surfaces with Pre-Ca were characterized after 1, 2, 4, 8 and 16 h of immersion in SCS by means of scanning electron microscopy together with energy dispersive X-ray analysis, X-ray diffraction and infrared absorption analysis. It was observed that the CPL precipitation rate with Pre-Ca averaged 1 microm h-1, twice as fast as without Pre-Ca. No precipitation was observed on untreated titanium with Pre-Ca up to day 14 of immersion in the SCS.

摘要

我们团队开发了一种简单的两步化学处理方法，即用盐酸和硫酸蚀刻，然后浸入沸腾的稀氢氧化钠溶液中，以制备具有生物活性的微孔钛表面，从而使磷酸钙层（CPL）能够从体外过饱和钙化溶液（SCS）中快速沉积。在这项工作中，采用了预钙化（Pre-Ca）程序，即将经过两步处理的钛先浸泡在磷酸氢二钠溶液中，然后再浸泡在饱和氢氧化钙溶液中，之后再浸入SCS中，以进一步加速CPL的沉淀。将经过Pre-Ca处理的钛表面在SCS中浸泡1、2、4、8和16小时后，通过扫描电子显微镜结合能量色散X射线分析、X射线衍射和红外吸收分析对其进行表征。结果观察到，经过Pre-Ca处理的CPL沉淀速率平均为1微米/小时，是未经Pre-Ca处理的两倍。在SCS中浸泡长达14天，未经处理的钛表面即使经过Pre-Ca处理也未观察到沉淀现象。