Collares-Buzato C B, Jepson M A, McEwan G T, Hirst B H, Simmons N L
Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, Newcastle upon Tyne, NE2 4HH, UK.
Cell Tissue Res. 1998 Feb;291(2):267-76. doi: 10.1007/s004410050996.
Distinct epithelial MDCK cell strains displaying extremes in transepithelial electrical resistance (paracellular permeability) have been established in co-culture and the subsequent cellular behaviour and formation of junctional complexes investigated. After high-density seeding, MDCK strain I and II cells in co-culture are initially randomly distributed but subsequently sort themselves out in a time-dependent manner to form separate homotypic aggregates. The final pattern of cell arrangement of homotypic aggregates depends on the relative seeding proportion of each cell type. Immunostaining of established marker proteins for junctional complexes has revealed that MDCK I and II cells differ in the degree of expression of the zonula-adherens-associated protein, E-cadherin, their cytoskeletal architecture and the junctional distribution of a desmosomal protein, and by showing subtle differences in tight junction staining for the zona-occludens-associated proteins, ZO-1 and occludin. The distinct pattern of junctional protein expression is maintained when the two MDCK strains are co-cultured; however, morphologically atypical intercellular junctions between heterotypic cells at the boundary of homotypic cell aggregates have been observed. It has been suggested that cell sorting, a phenomenon yet to be completely understood, is involved in important morphogenetic processes. We propose that co-culture of strains of the well-characterised MDCK cell line may be a novel but well-defined cell system for studying epithelial cell rearrangement and sorting in intact epithelial sheets.
已经通过共培养建立了具有跨上皮电阻（细胞旁通透性）极端差异的不同上皮MDCK细胞株，并对随后的细胞行为和连接复合体的形成进行了研究。高密度接种后，共培养中的MDCK I型和II型细胞最初随机分布，但随后会以时间依赖性方式自行分类，形成单独的同型聚集体。同型聚集体的最终细胞排列模式取决于每种细胞类型的相对接种比例。对连接复合体的既定标记蛋白进行免疫染色显示，MDCK I型和II型细胞在黏着小带相关蛋白E-钙黏蛋白的表达程度、细胞骨架结构以及桥粒蛋白的连接分布方面存在差异，并且在紧密连接染色中，对于紧密连接相关蛋白ZO-1和闭合蛋白也表现出细微差异。当两种MDCK细胞株共培养时，连接蛋白表达的独特模式得以维持；然而，在同型细胞聚集体边界处的异型细胞之间观察到了形态上非典型的细胞间连接。有人提出，细胞分选这一尚未完全理解的现象参与了重要的形态发生过程。我们认为，对特征明确的MDCK细胞系的不同细胞株进行共培养，可能是一种用于研究完整上皮片中上皮细胞重排和分选的新颖且明确的细胞系统。
