Yaqoob M, Nabi A, Masoom-Yasinzai M
Department of Chemistry, University of Balochistan, Quetta, Pakistan.
J Biolumin Chemilumin. 1997 May-Jun;12(3):135-40. doi: 10.1002/(SICI)1099-1271(199705/06)12:3<135::AID-BIO439>3.0.CO;2-B.
A flow injection chemiluminescent method is described for the determination of choline. The method is based on the production of hydrogen peroxide from choline using on-line covalently bound immobilized choline oxidase column. The product is mixed downstream and detected via the cobalt catalyzed chemiluminescent oxidation of luminol. The detection limit is 1 x 10(-7) mol/L, with rsd 1.8 to 2.8% in the range 2-10 x 10(-5) mol/L. The sample throughput is 30 per hour. The method was applied to the determination of choline produced off-line from phosphatidylcholine using phospholipase-D isolated from cabbage.
