Martins A P, Marras R A, Guimarães F S
Department of Pharmacology, FMRP, Campus USP, Ribeirão Preto, Brazil.
Neuroreport. 1997 Nov 10;8(16):3601-4. doi: 10.1097/00001756-199711100-00036.
To investigate whether the dorsal periaqueductal grey (DPAG) might be involved in the anxiogenic effect of intracerebroventricular (i.c.v.) administered corticotropin-releasing hormone (CRH), rats (n = 6-13) received microinjections into the DPAG of CRH (1 or 2 microg) or saline and were tested on the elevated plus maze. The drug caused a dose-dependent decrease in plus maze exploration (percentage of entries into open arms: saline 31.9 +/- 8.6, CRH 1 microg 19.2 +/- 4.0, CRH 2 microg 6.9 +/- 3.7; p < 0.01, ANOVA). In a second experiment the anxiogenic effect of intra-DPAG CRH (2 microg) was prevented by previous microinjection of alpha-helical-CRH(9-41) (0.5 microg), a CRH antagonist (percentage of entries into open arms: saline + CRH 20.3 +/- 3.7, alpha-helical-CRH(9-41) + CRH 45.7 +/- 1.6). These results suggest that the DPAG may be a site of the anxiogenic effect of i.c.v. injected CRH.
为研究中脑导水管周围灰质背侧（DPAG）是否参与脑室内（i.c.v.）注射促肾上腺皮质激素释放激素（CRH）的致焦虑作用，将大鼠（n = 6 - 13）分为两组，分别向其DPAG内微量注射CRH（1或2微克）或生理盐水，然后在高架十字迷宫中进行测试。药物导致十字迷宫探索行为呈剂量依赖性减少（进入开放臂的百分比：生理盐水组31.9 +/- 8.6，CRH 1微克组19.2 +/- 4.0，CRH 2微克组6.9 +/- 3.7；p < 0.01，方差分析）。在第二个实验中，预先微量注射CRH拮抗剂α-螺旋-CRH(9 - 41)（0.5微克）可预防DPAG内注射CRH（2微克）的致焦虑作用（进入开放臂的百分比：生理盐水 + CRH组20.3 +/- 3.7，α-螺旋-CRH(9 - 41) + CRH组45.7 +/- 1.6）。这些结果表明，DPAG可能是i.c.v.注射CRH致焦虑作用的一个位点。
