Poteryaev D A, Zakharov I S, Balaban P M, Uvarov P N, Belyavsky A V
Laboratory of Molecular Bases of Differentiation and Development, Engelhardt Institute of Molecular Biology, Moscow, Russia.
Neuroreport. 1997 Nov 10;8(16):3631-5. doi: 10.1097/00001756-199711100-00041.
We report the isolation of a Helix lucorum cDNA clone encoding a precursor of neuropeptides that are closely related to Aplysia and Tritonia pedal peptides (Pep). The predicted propeptide contains 20 copies of the two variants of Helix Pep interspersed with Lys-Arg endopeptidase cleavage sites. Northern blot hybridization revealed multiple Pep-hybridizing species in the Helix CNS RNA. The Pep gene was expressed by several identified serotonergic neurones in pedal and cerebral ganglia, groups of sensory neurones in procerebrum, peripheral neurones in olfactory bulb, mantle and foot, and group of neurones in pedal ganglia presumably involved in locomotion control. Pep mRNA was detected in several neurones at the early stages of nervous system development.
我们报道了一种福寿螺cDNA克隆的分离，该克隆编码与海兔和海神鳃踏板肽（Pep）密切相关的神经肽前体。预测的前体肽包含20个福寿螺Pep两种变体的拷贝，其间穿插着赖氨酰-精氨酰内肽酶切割位点。Northern印迹杂交显示福寿螺中枢神经系统RNA中有多个与Pep杂交的物种。Pep基因在足神经节和脑神经节中的几个已鉴定的5-羟色胺能神经元、前脑的感觉神经元群、嗅球、外套膜和足部的外周神经元以及可能参与运动控制的足神经节中的神经元群中表达。在神经系统发育的早期阶段，在几个神经元中检测到了Pep mRNA。
