Krüger M, Fischer R
Philipps Universität Marburg, Laboratorium für Mikrobiologie and Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany.
EMBO J. 1998 Jan 2;17(1):204-14. doi: 10.1093/emboj/17.1.204.
Genetic features determine the site of polarized growth in filamentous fungi and lead to hyphal tip extension or subapical branching. We have isolated the samB gene (suppressor of anucleate metulae) of Aspergillus nidulans which encodes a 66 kDa protein carrying an atypical Cys4 and an additional Cys2/His/Cys Zn finger motif at the carboxy-terminus. Such novel Zn finger-like domains have recently been found in several other developmental regulators in organisms ranging from yeast to man. Deletion of this domain at the carboxy-terminus of SamB led to premature hyphal ramification, mislocalization of septa and suppression of the asporogenous phenotype of the developmental mutant aps (anucleate primary sterigmata). A DeltasamB deletion strain displayed an identical phenotype. A homologous gene in Saccharomyces cerevisiae was also characterized whose deletion resulted in a multi-budding phenotype; thus it was named MUB1. An underlying common mechanism for both genes in determination of the onset of polarized growth and its links to other cellular developmental processes is discussed.
遗传特征决定丝状真菌中极性生长的位点，并导致菌丝顶端延伸或亚顶端分支。我们分离了构巢曲霉的samB基因（无核小梗的抑制子），该基因编码一种66 kDa的蛋白质，在其羧基末端带有一个非典型的Cys4和一个额外的Cys2/His/Cys锌指基序。最近在从酵母到人类的多种生物的其他几种发育调节因子中也发现了这种新型的锌指样结构域。删除SamB羧基末端的这个结构域会导致菌丝过早分支、隔膜定位错误以及发育突变体aps（无核初级小梗）的无孢子表型受到抑制。一个ΔsamB缺失菌株表现出相同的表型。酿酒酵母中的一个同源基因也被鉴定出来，其缺失导致多芽表型，因此被命名为MUB1。本文讨论了这两个基因在确定极性生长起始及其与其他细胞发育过程的联系方面的潜在共同机制。
