Lundblad R L, Bradshaw R A
Baxter Biotech-Hyland Division, Duarte, CA 91010, USA.
Biotechnol Appl Biochem. 1997 Dec;26(3):143-51.
The chemical modification of proteins has long been a useful approach to elucidating structure-function relationships. Recently, these same chemistries have been finding application in the preparation of proteins for both diagnostic and therapeutic use. These applications include alterations to introduce new properties, such as improved stability, or new traits, such as drug binding and transport, that take advantage of both broad and narrow ranges of selectivity. Site-specific chemical modification of proteins, when combined with the powerful advantages of site-specific mutagenesis, can yield protein agents superior to those generated by either approach alone.
长期以来，蛋白质的化学修饰一直是阐明结构 - 功能关系的一种有用方法。最近，这些相同的化学方法已在制备用于诊断和治疗的蛋白质中得到应用。这些应用包括引入新特性（如提高稳定性）或新特征（如药物结合和转运）的改变，这些改变利用了广泛和狭窄的选择性范围。蛋白质的位点特异性化学修饰，与位点特异性诱变的强大优势相结合，可以产生优于单独使用任何一种方法所产生的蛋白质药物。
