采用同步和序贯荧光原位杂交技术进行细胞遗传学分析。
Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization.
作者信息
Escudero T, Fuster C, Coll M D, Egozcue J
机构信息
Department de Biologia cel-lular i Fisiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
出版信息
Cancer Genet Cytogenet. 1998 Jan 15;100(2):111-3. doi: 10.1016/s0165-4608(97)00029-0.
PMID:9428353
Abstract
A sequential fluorescence in situ hybridization (FISH) technique is described. This method allows the detection of up to eighteen chromosome pairs in consecutive hybridizations (8 steps) on the same metaphase using centromeric, whole chromosome painting, and single copy DNA probes with different fluorochromes. The technique may be used with diagnostic purposes in cases with poor cytogenetic material.
摘要
本文描述了一种连续荧光原位杂交（FISH）技术。该方法能够在同一中期细胞上连续进行杂交（8个步骤），使用着丝粒、全染色体涂染和带有不同荧光染料的单拷贝DNA探针，检测多达18对染色体。该技术可用于细胞遗传学材料较差的病例的诊断。
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