Flores G, Martinez F, Reyes P A, Cortes J J
Departamento de Bioquímica Molecular, Centro de Biología Experimental, Universidad Autónoma de Zacatecas, Guadalupe, México.
Arch Med Res. 1997 Winter;28(4):571-5.
Immunoprecipitation analysis of total HeLa cells RNA extract by protein A-Sheparose purified autoantibodies and pCp 32P-3' end labeling RNAs revealed that U1, U2, U4 and U5 snRNAs are related with anti-Sm or U1nRNP autoantibodies, while the hY1, hY3, hY4 and hY5 scRNAs were related to anti-SSA/Ro autoantibodies present in sera of patients with Systemic Lupus Erythematosus. The authors detected molecular snRNAs and scRNAs specificities by autoantibodies in 71 sera, the molecular RNA specificity for anti-Sm (U1, U2, U4 and U5 snRNAs) was present in 39%; anti-SSA/Ro sera reacted against scRNAs (hY1, hY3, hY4 and hY5) in 36%, then anti-U1nRNP sera recognized U1 snRNA in 13% of sera and anti-rRNP related with rRNA were recognized in 8%. Twenty-nine SLE sera were RNA negative. A molecular characterization of the autoantibodies in sera from SLE patients may be a useful tool for clinical and laboratory diagnosis of SLE, and the use of autoantibodies as molecular probes allows to continue exploring some basic mechanism of gene expression.
用蛋白A - 琼脂糖纯化的自身抗体对HeLa细胞总RNA提取物进行免疫沉淀分析，并用pCp 32P - 3'末端标记RNA，结果显示U1、U2、U4和U5 snRNA与抗Sm或U1nRNP自身抗体相关，而hY1、hY3、hY4和hY5 scRNA与系统性红斑狼疮患者血清中存在的抗SSA/Ro自身抗体相关。作者在71份血清中通过自身抗体检测到分子snRNA和scRNA特异性，抗Sm（U1、U2、U4和U5 snRNA）的分子RNA特异性存在于39%的血清中；抗SSA/Ro血清与scRNA（hY1、hY3、hY4和hY5）反应的占36%，抗U1nRNP血清在13%的血清中识别U1 snRNA，与rRNA相关的抗rRNP在8%的血清中被识别。29份SLE血清RNA呈阴性。对SLE患者血清中自身抗体进行分子特征分析可能是SLE临床和实验室诊断的有用工具，并且将自身抗体用作分子探针有助于继续探索基因表达的一些基本机制。
