中国仓鼠卵巢（CHO-K1）细胞中DNA复制的多个亚阶段。

Multiple subphases of DNA replication in Chinese hamster ovary (CHO-K1) cells.

作者信息

Banfalvi G, Mikhailova M, Poirier L A, Chou M W

机构信息

Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University Medical School, Budapest, Hungary.

出版信息

DNA Cell Biol. 1997 Dec;16(12):1493-8. doi: 10.1089/dna.1997.16.1493.

DOI:10.1089/dna.1997.16.1493

PMID:9428798

Abstract

Replicative DNA synthesis has been measured throughout the S phase in synchronized populations of Chinese hamster ovary cells. When exponentially growing, cells in suspension cultures were subjected to counterflow centrifugal elutriation and the resolution power was increased the biphasic replication profile has been resolved and multiple subphases were distinguished. These replication peaks, termed replication checkpoints, are distributed evenly throughout the S phase. The replication checkpoints have been characterized by their average C values corresponding to 2.05, 2.12, 2.2, 2.45, 2.6, 2.8, 2.95, 3.15, 3.3, 3.45, and 3.85.

摘要

已在中国仓鼠卵巢细胞同步群体的整个S期测量了复制性DNA合成。当指数生长时，悬浮培养的细胞进行逆流离心淘析，分辨率提高，双相复制谱得以解析，并区分出多个亚阶段。这些复制峰，称为复制检查点，在整个S期均匀分布。复制检查点已根据其平均C值进行了表征，这些值分别对应于2.05、2.12、2.2、2.45、2.6、2.8、2.95、3.15、3.3、3.45和3.85。