Lu Y, Lin J, Li S, Li X, Shu Y, Cen P
Department of Chemical Engineering, Zhejiang University, Hangzhou, China.
Chin J Biotechnol. 1997;13(3):169-76.
The Rhizopus oryzae was immobilized by the calcium alginate entrapment method. A three-phase fluidized-bed bioreactor was designed to perform the immobilized-cell L-lactic acid fermentation. A solvent extraction column was coupled with the bioreactor to remove L-lactic acid from the fermentation broth. The TRPO was selected as solvent and sulfonated kerosene as diluent. The results indicated that the pH value in the broth was regulated above pH 3.5 and the fermentation rate was as high as 11 g L-lactic acid per hour per liter of beads. A mathematical model was proposed to describe the concentration of L-lactic acid in the extractive fermentation.
米根霉通过海藻酸钙包埋法固定化。设计了一种三相流化床生物反应器用于进行固定化细胞L-乳酸发酵。将一个溶剂萃取柱与生物反应器相连，以从发酵液中去除L-乳酸。选择三烷基氧膦（TRPO）作为溶剂，磺化煤油作为稀释剂。结果表明，发酵液的pH值调节至pH 3.5以上，发酵速率高达每升珠子每小时11 g L-乳酸。提出了一个数学模型来描述萃取发酵中L-乳酸的浓度。
