Huang Y, Qiao C, Williamson M S, Devonshire A L
State Key Lab of Integrated Management of Pest Insects and Rodents, Institute of Zoology, CAS, Beijing, China.
Chin J Biotechnol. 1997;13(3):177-83.
Acetylcholinesterase (AChE) is the target site for the organophosphates and carbamates in insects. Widespread use of these two classes of insecticides has led to the selection of resistance. Target modification was regarded as a molecular mechanism in some resistance species. The altered AChEs with reduced sensitivity to inhibition are related to this resistance. AChE genes from two insecticide-resistant housefly (Musca domestica) strains D3 and Kash were isolated and sequenced using RT-PCR and streptavidin-linked magnetic bead techniques. The cDNAs have a 2082-bp open reading frame from which the complete amino acid sequence of AChE has been deduced. Some differences in nucleotide sequence and four-point mutations of amino acid were found compared to a susceptible strain, i.e., the Cooper strain. Three substitutions are likely to confer insecticide insensitivity, which seems that D3 and Kash belong to CH2 pattern of resistance.
乙酰胆碱酯酶（AChE）是昆虫体内有机磷酸酯类和氨基甲酸酯类杀虫剂的作用靶标位点。这两类杀虫剂的广泛使用导致了昆虫抗药性的产生。靶标修饰被认为是一些抗性昆虫的分子机制。对抑制作用敏感性降低的AChE改变与这种抗性有关。利用逆转录聚合酶链式反应（RT-PCR）和链霉亲和素连接磁珠技术，从两个抗杀虫剂家蝇（Musca domestica）品系D3和Kash中分离并测序了AChE基因。这些cDNA具有一个2082bp的开放阅读框，由此推导了AChE完整的氨基酸序列。与敏感品系即库珀品系相比，发现核苷酸序列存在一些差异以及四个氨基酸点突变。三个替换可能导致对杀虫剂不敏感，这似乎表明D3和Kash属于CH2抗性模式。
