Puchkov V F, Popov V B
Arkh Anat Gistol Embriol. 1976 Jan;70(1):78-84.
To reveal the developing vessels of the yolk placenta the erythrocytes were stained with benzidine. The development of vessels was studied in vivo and in transplantation of embryos in the homologous blood serum. The pattern of branching of blood vessels in the yolk sac of 12-day embryos was established. The development of the blood circulatory system started on the 9,5 day (the anlage of sanguineous islands), the definitive state was acquired by the 12th day. The development of vessels in vitro after removal of the parietal layer of the placenta is dependent on the mophological stage at the moment of transplantation. In explantation of embryos with 2--3 pairs of somites which are being already formed, the yolk vessels develop as those in control. In transplantation of presomite embryos the development of vessels is sharply disturbed, in transplantation of embryos of the egg cylinder stage the vessels are not developed. The inducing role of the yolk placenta perietal layer in the development of blood vessels in the vascular layer is supposed.
为了揭示卵黄胎盘发育中的血管，用联苯胺对红细胞进行染色。在体内以及将胚胎移植到同源血清中研究血管的发育。确定了12天胚胎卵黄囊中血管的分支模式。血液循环系统在第9.5天开始发育（血岛原基），在第12天达到最终状态。去除胎盘壁层后体外血管的发育取决于移植时的形态学阶段。在移植已经形成2 - 3对体节的胚胎时，卵黄血管的发育与对照相同。在移植前体节胚胎时，血管发育受到严重干扰，在移植卵柱期胚胎时，血管不发育。推测卵黄胎盘壁层在血管层血管发育中具有诱导作用。
