Moulki H, Bonaly R, Fournet B, Montreuil J
Biochim Biophys Acta. 1976 Feb 20;420(2):279-87. doi: 10.1016/0005-2795(76)90319-6.
Following ethylene diamine treatment of the cell wall of Rhodotorula rubra, a water soluble fraction has been isolated. This fraction can be resolved into three glycoproteins and one protein. The major part is a glycoprotein, purified to homogeneity which has a molecular weight of 64 000. The glyco-part of this protein contains mannose, glucose and one residue of glucosamine. After pronase treatment, the presence of an ""Asparaginyl-N-acetylglucosamine" linkage is established by the existence of one aspartic acid residue and one glucosamine residue. After permethylation, the initial data give some evidence that the branching points in the molecule were the carbon atoms 3 and (or) 6 of the mannose moiety and that some glucose moieties are bound to the non-reducing terminal end.
在用乙二胺处理深红酵母细胞壁后，分离出了一种水溶性组分。该组分可分解为三种糖蛋白和一种蛋白质。主要部分是一种糖蛋白，已纯化至同质，其分子量为64000。这种蛋白质的糖部分含有甘露糖、葡萄糖和一个氨基葡萄糖残基。经链霉蛋白酶处理后，通过一个天冬氨酸残基和一个氨基葡萄糖残基的存在确定了“天冬酰胺基 - N - 乙酰葡糖胺”键的存在。经全甲基化后，初步数据提供了一些证据，表明分子中的分支点是甘露糖部分的碳原子3和（或）6，并且一些葡萄糖部分与非还原末端相连。
