Hunt W L, Foote R H
Department of Animal Science, Cornell University, Ithaca, New York 14853-4801, USA.
J Androl. 1997 Nov-Dec;18(6):740-4.
The study was designed to quantitatively evaluate the effect of repeated testicular biopsy of avascular areas by the open method on spermatogenesis, semen quality, and sperm output in dogs. After a 5-week standardization period of semen collections 3x per week, 10 sexually mature Beagle dogs were divided into two groups, approximately equalized based on previous sperm output. Semen collections were continued for 20 weeks. One group was controls without treatment until unilaterally orchidectomized after weeks 16 and 20; a second group had one testis biopsied on weeks 1, 5, and 9 and removed after week 16, with the second testis removed after week 20. Sperm output per week during the summer declined in the control group from 851 x 10(6) to 725 x 10(6) (15%). The decline in the biopsied control group collected simultaneously was 22%, presumably reflecting a seasonal effect plus a possible small treatment effect. At 16 weeks, the testes removed from the controls averaged 7.1 g and the testes biopsied three times in the second group averaged 6.4 g (P > 0.05). Scrotal-testicular measurements taken in live animals were correlated with testicular weights at orchidectomy (r = 0.81). An average of 36.7 mg of tissue was removed at each biopsy, sufficient to evaluate 100 to >200 seminiferous tubules in cross-section. Sperm motility and frequency of all stages of the cycle of the seminiferous epithelium were similar between control and biopsied dogs. It is concluded that repeated testicular biopsy of avascular areas can provide sufficient tissue for histopathologic evaluation without significant interference with spermatogenesis.
本研究旨在定量评估开放式方法对犬无血管区域进行重复睾丸活检对精子发生、精液质量和精子产量的影响。在每周收集3次精液的5周标准化期后，10只性成熟的比格犬被分为两组，根据先前的精子产量大致均衡。精液收集持续20周。一组为对照组，在第16周和第20周进行单侧睾丸切除前不进行治疗；另一组在第1、5和9周对一侧睾丸进行活检，并在第16周后切除，另一侧睾丸在第20周后切除。对照组夏季每周的精子产量从851×10⁶降至725×10⁶（下降15%）。同期进行活检的对照组下降了22%，这可能反映了季节效应以及可能的微小治疗效应。在第16周时，对照组切除的睾丸平均重7.1克，第二组经三次活检的睾丸平均重6.4克（P>0.05）。活体动物阴囊-睾丸测量值与睾丸切除时的睾丸重量相关（r=0.81）。每次活检平均切除36.7毫克组织，足以评估100至200多个横切的生精小管。对照组和活检组犬的精子活力以及生精上皮周期各阶段的频率相似。结论是，对无血管区域进行重复睾丸活检可为组织病理学评估提供足够的组织，而不会对精子发生造成显著干扰。
