苯基硫脲对罗氏白绢病菌和核盘菌生长及菌核形成的影响。

Effects of phenylthiourea on growth and sclerotial formation of Sclerotium rolfsii and Whetzelinia sclerotiorum.

作者信息

Le Tourneau D

出版信息

Can J Microbiol. 1976 Mar;22(3):379-83. doi: 10.1139/m76-057.

Abstract

The growth of Sclerotium rolfsii and Whetzelinia sclerotiorum was reduced when 5 X 10(-4) to 2 X 10(-3) M 1-phenyl-2-thiourea (PTU) was incorporated into synthetic media and potato dextrose agar (PDA). Whetzelinia sclerotiorum produced heavy aerial mycelia and few, if any, sclerotia in synthetic glucose-nitrate liquid medium containing 10(-3) and 2 X 10(-3) M PTU. At the same PTU concentrations in PDA. W. sclerotiorum formed abnormal sclerotia covered with a yellowish green exudate. Sclerotium rolfsii produced unusual patterns of aerial mycelia and no sclerotia on media containing 2 X 10(-3) M PTU. With 5 X 10(-4) M PTU, S. rolfsii produced sclerotial initials and some of these developed into atypical clumps of sclerotia. A yellow pigment developed when S. rolfsii grew on media containing PTU.

摘要

当在合成培养基和马铃薯葡萄糖琼脂（PDA）中加入5×10⁻⁴至2×10⁻³M的1-苯基-2-硫脲（PTU）时，齐整小核菌和核盘菌的生长受到抑制。在含有10⁻³和2×10⁻³M PTU的合成葡萄糖-硝酸盐液体培养基中，核盘菌产生大量气生菌丝体，几乎不产生菌核（如果有也是极少）。在PDA中相同PTU浓度下，核盘菌形成覆盖有黄绿色渗出物的异常菌核。在含有2×10⁻³M PTU的培养基上，齐整小核菌产生异常的气生菌丝体模式且不产生菌核。在含有5×10⁻⁴M PTU时，齐整小核菌产生菌核原基，其中一些发育成非典型的菌核团块。当齐整小核菌在含有PTU的培养基上生长时会产生一种黄色色素。