Galan P, Preziosi P, Monget A L, Richard M J, Arnaud J, Lesourd B, Girodon F, Alferez M J, Bourgeois C, Keller H, Favier A, Hercberg S
Institut Scientifique et Technique de la Nutrition et de l'Alimentation, CNAM, Paris, France.
Int J Vitam Nutr Res. 1997;67(6):450-60.
A randomized double-blind trial was performed in order to assess the efficacity of differing combinations of antioxidant nutrients on biochemical parameters of vitamin and trace element status, immunological parameters and free radical metabolism in elderly long term hospitalized subjects. A total of 756 institutionalized elderly subjects were recruited in 26 nursing homes in different areas of France. Four groups were constituted, receiving daily, for 1 year, either vitamins (beta-carotene, 6 mg; vitamin C, 120 mg; and vitamin E, 15 mg), trace elements (zinc, 20 mg and selenium, 100 micrograms), trace elements associated with vitamins, or a placebo. Biochemical indicators of trace elements and vitamin status and free radical parameters were measured before and after 6 months and 1 year of supplementation. Some immunological markers were investigated initially and after 6 months of supplementation on a subsample of 134 subjects. Mean plasma levels of alpha-tocopherol, gamma-tocopherol, vitamin C, alpha-carotene, beta-carotene and copper increased significantly after 6 months of supplementation in groups receiving vitamins alone or associated with trace elements. Serum selenium concentrations were significantly increased at 6 months of supplementation, and serum zinc only after one year in the trace element groups. Serum lycopene levels were significantly decreased by trace element supplementation. A significant increase in Se-glutathione peroxidase (GPx) levels was observed in groups receiving trace elements alone or associated with vitamins. No effect was noted on superoxide dismutase (SOD) activity or TBARs production. No effect of supplementation was found for in vitro lymphocyte proliferative responses or most lymphocyte subsets, except for a significantly lower percentage of CD2 subsets observed in groups receiving mineral supplementation either alone or associated with vitamins. A significant difference in CD19 subsets was found in groups receiving trace elements. Mean IL-1 production was significantly higher after 6 months of supplementation in the vitamin groups.
为了评估抗氧化营养素的不同组合对长期住院老年受试者维生素和微量元素状态的生化参数、免疫参数及自由基代谢的功效，进行了一项随机双盲试验。在法国不同地区的26家养老院招募了总共756名机构养老的老年受试者。组成了四组，分别每日接受为期1年的维生素（β-胡萝卜素6毫克、维生素C 120毫克和维生素E 15毫克）、微量元素（锌20毫克和硒100微克）、与维生素联合的微量元素或安慰剂。在补充6个月和1年后，测量微量元素和维生素状态的生化指标以及自由基参数。对134名受试者的子样本在补充开始时及6个月后研究了一些免疫标志物。单独接受维生素或联合微量元素的组在补充6个月后，血浆α-生育酚、γ-生育酚、维生素C、α-胡萝卜素、β-胡萝卜素和铜的平均水平显著升高。补充6个月时血清硒浓度显著升高，而微量元素组中血清锌仅在1年后升高。补充微量元素使血清番茄红素水平显著降低。单独接受微量元素或联合维生素的组中，硒谷胱甘肽过氧化物酶（GPx）水平显著升高。未观察到对超氧化物歧化酶（SOD）活性或丙二醛（TBARs）生成有影响。补充对体外淋巴细胞增殖反应或大多数淋巴细胞亚群没有影响，除了单独接受矿物质补充或联合维生素的组中观察到CD2亚群的百分比显著降低。接受微量元素的组中CD19亚群存在显著差异。维生素组在补充6个月后平均白细胞介素-1生成显著更高。
