Lilley K G, Epping R J, Hafner L M
Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, Brisbane, Australia.
Lab Anim Sci. 1997 Dec;47(6):632-7.
Immunologic responses elicited after vaginal immunization of laboratory mammals are affected by the stage of the animal's estrous cycle at which antigen is delivered. The study reported here documents vaginal impedance as an improved means of determining accurately precise stages of the estrous cycle of guinea pigs. Impedance changes were correlated with cytologic changes observed in vaginal smears taken from the animals. The start of the impedance peak corresponded with proestrus, the rising side of the peak with estrus, and the apex of the peak with metestrus; diestrus was seen approximately 3 days after the peak apex. These results contrast with those of previous studies. Various dosage regimens of estradiol (1 to 1,000 micrograms/animal over 1 to 6 days) were used in guinea pigs in an attempt to invoke an extended (> 6 h) estrogen-induced mucosa. Parenteral administration of 1,000 micrograms of estradiol to guinea pigs daily for 6 days induced a 2- to 3-day estrus stage in these animals.
实验室哺乳动物经阴道免疫后引发的免疫反应会受到抗原递送时动物发情周期阶段的影响。本文报道的研究记录了阴道阻抗作为一种更精确确定豚鼠发情周期阶段的改进方法。阻抗变化与从动物采集的阴道涂片观察到的细胞学变化相关。阻抗峰值的开始对应于发情前期，峰值的上升阶段对应于发情期，峰值的顶点对应于发情后期；发情间期大约在峰值顶点后3天出现。这些结果与先前研究的结果不同。在豚鼠中使用了各种雌二醇剂量方案（1至6天内每只动物1至1000微克），试图诱导延长（>6小时）的雌激素诱导黏膜。给豚鼠每天皮下注射1000微克雌二醇，持续6天，在这些动物中诱导出2至3天的发情期阶段。
