Eisenstein Z, Boruchowsky S, Bank H, Czerniak P
Isr J Med Sci. 1976 Jan;12(1):1-10.
An accurate, highly sensitive triiodothyrone (T3) radioimmunoassay system is described. Direct measurement in serum is made possible by the use of T3-free serum for standards, and blocking of T3 binding to thyroxine (T4)-binding globulin with salicylate. T4 cross reactivity was less than 0.3%. Mean T3 levels in 55 euthyroid, 32 hyperthyroid and 19 primary hypothyroid patients were 1.46 +/- 0.17, 5.34 +/- 1.86 and 0.49 +/- 0.34 (SD) ng/ml, respectively. T3 was found to be a more sensitive index than T4 in hyperthyroidism; in hypothyroidism it overlapped with euthyroidism. The presence of goiter had no effect on T3 concentrations in euthyroid patients. Among the patients studied, eight were found to have T3 toxicosis, including two patients with recurrent thyrotoxicosis. A significant T3 elevation was also found in euthyroid patients after thyroidectomy whereas T4 remained normal. The extent of T4 and T3 elevation was similar during the initial phase of subacute thyroiditis. There were significantly lower T3 values and higher thyroid-stimulating hormone values in umbilical cord blood than in maternal blood. In two euthyroid subjects, thyrotropin-releasing hormone stimulation caused a 50% rise in T3 levels and no change in T4 levels over a 2-h period.
本文描述了一种准确、高度灵敏的三碘甲状腺原氨酸（T3）放射免疫分析系统。使用不含T3的血清作为标准品，并利用水杨酸盐阻断T3与甲状腺素（T4）结合球蛋白的结合，从而实现血清中T3的直接测量。T4交叉反应率小于0.3%。55例甲状腺功能正常、32例甲状腺功能亢进和19例原发性甲状腺功能减退患者的平均T3水平分别为1.46±0.17、5.34±1.86和0.49±0.34（标准差）ng/ml。在甲状腺功能亢进症中，T3被发现是比T4更敏感的指标；在甲状腺功能减退症中，它与甲状腺功能正常状态有重叠。甲状腺肿的存在对甲状腺功能正常患者的T3浓度没有影响。在研究的患者中，发现8例患有T3中毒，包括2例复发性甲状腺毒症患者。甲状腺切除术后甲状腺功能正常的患者也发现T3显著升高，而T4保持正常。亚急性甲状腺炎初始阶段T4和T3升高的程度相似。脐带血中的T3值显著低于母血，促甲状腺激素值则高于母血。在两名甲状腺功能正常的受试者中，促甲状腺激素释放激素刺激导致T3水平在2小时内升高50%，T4水平无变化。
