Alarcón Corredor O M, Silva Larralte T, Chacón Patiño A, Pachano Primera Y J, Alarcón A O, Reinosa J
Departamento de Bioquímica, Facultad de Medicina. Universidad de Los Andes, Mérida, Venezuela.
Acta Cient Venez. 1996;47(2):127-31.
In this study, the serum, urinary, hepatic and renal levels of total inorganic phosphorus (Pi) were determined in guinea pigs treated with 100,000 U.I. of vitamin A, dissolved in Tween Span, given each day by intramuscular injections during seven days (treated group), and compared with the levels in healthy guinea pigs which were given the same feed as the treated group, and received the same amount of Tween Span (control group). The activity of the enzymes alkaline phosphatase and acid phosphatase was simultaneously determined in liver and kidneys of both groups, and the phospholipid content in serum and tissue was also determined. The treated group did not show changes in serum and urinary phosphorus as compared with the control group. Liver and kidney of the treated group showed an increase in total Pi (p < 0.05). In this group a definite increase (p < 0.05) was also observed both in serum and tissue levels of phospholipids, as well as in the activity of the liver and kidney enzymes studied. These results suggest that phosphorus enters the blood stream in the form of lipid complexes (phospholipids) that are involved in certain characteristic functions.
在本研究中，测定了用100,000国际单位维生素A（溶于吐温司盘）每天肌肉注射7天的豚鼠（治疗组）血清、尿液、肝脏和肾脏中总无机磷（Pi）的水平，并与给予与治疗组相同饲料且接受相同量吐温司盘的健康豚鼠（对照组）的水平进行比较。同时测定了两组肝脏和肾脏中碱性磷酸酶和酸性磷酸酶的活性，并测定了血清和组织中的磷脂含量。与对照组相比，治疗组血清和尿液磷未显示变化。治疗组的肝脏和肾脏总Pi增加（p < 0.05）。在该组中，血清和组织磷脂水平以及所研究的肝脏和肾脏酶的活性也均有明显增加（p < 0.05）。这些结果表明，磷以参与某些特征性功能的脂质复合物（磷脂）形式进入血流。
