Noma T, Song S, Yoon Y S, Tanaka S, Nakazawa A
Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan.
Biochim Biophys Acta. 1998 Jan 7;1395(1):34-9. doi: 10.1016/s0167-4781(97)00193-0.
We isolated two kinds of cDNAs encoding human adenylate kinase (AK) isozyme 2 from a HeLa cell cDNA library using bovine AK2 cDNA as a probe. Nucleotide sequencing revealed that the cDNAs encoded 239- and 232-amino acid proteins with deduced molecular mass of 26.5 (AK2A) and 25.6kDa (AK2B), respectively. Northern blot analysis demonstrated that AK2 mRNA is strongly expressed in liver, heart, skeletal muscle and pancreas, and moderately in kidney, placenta and brain, and weakly in lung. However, Western blot analysis showed that AK2 protein was present in large amounts in liver, heart, kidney, and in a small amount in lung, and undetectable in brain and skeletal muscle. These results suggested the presence of the tissue-specific gene-expression including post-transcriptional regulation in expression of the AK2 gene.
我们以牛腺苷酸激酶2（AK2）cDNA为探针，从HeLa细胞cDNA文库中分离出两种编码人腺苷酸激酶（AK）同工酶2的cDNA。核苷酸测序显示，这些cDNA分别编码239和232个氨基酸的蛋白质，推导分子量分别为26.5kDa（AK2A）和25.6kDa（AK2B）。Northern印迹分析表明，AK2 mRNA在肝脏、心脏、骨骼肌和胰腺中强烈表达，在肾脏、胎盘和大脑中中度表达，在肺中弱表达。然而，Western印迹分析显示，AK2蛋白在肝脏、心脏、肾脏中大量存在，在肺中少量存在，在大脑和骨骼肌中未检测到。这些结果提示AK2基因存在包括转录后调控在内的组织特异性基因表达。
