Hu P, Zhang K M, Spratt J A, Wechsler A S, Briggs F N
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298, USA.
Biochim Biophys Acta. 1998 Jan 7;1395(1):121-5. doi: 10.1016/s0167-4781(97)00135-8.
Chronic low frequency stimulation of predominantly fast-twitch skeletal muscles decrease the levels of SERCA1 (fast-twitch sarco(endo)plasmic reticulum Ca2+-ATPase) mRNA, and increase the levels of SERCA2 (slow-twitch sarco(endo)plasmic reticulum Ca2+-ATPase) and phospholamban mRNAs. To assess the role of transcription in these changes in mRNA levels, nuclei were isolated from chronically stimulated canine latissimus dorsi muscles and transcription rates were estimated by nuclear run-on assays. Decreases in the rates of SERCA1 gene transcription matched the fall in its mRNA level and increases in the rates of SERCA2 and phospholamban gene transcription matched the increases in their mRNAs.
对主要为快肌纤维的骨骼肌进行慢性低频刺激，会降低肌浆网/内质网Ca²⁺ - ATP酶1（快肌纤维肌浆网/内质网Ca²⁺ - ATP酶）mRNA的水平，并增加肌浆网/内质网Ca²⁺ - ATP酶2（慢肌纤维肌浆网/内质网Ca²⁺ - ATP酶）和受磷蛋白mRNA的水平。为了评估转录在这些mRNA水平变化中的作用，从慢性刺激的犬背阔肌中分离出细胞核，并通过核转录分析来估计转录速率。肌浆网/内质网Ca²⁺ - ATP酶1基因转录速率的降低与其mRNA水平的下降相匹配，而肌浆网/内质网Ca²⁺ - ATP酶2和受磷蛋白基因转录速率的增加与其mRNA水平的增加相匹配。
