本地生产的快速尿素酶试验与组织学检查在诊断幽门螺杆菌感染中的前瞻性比较。

A prospective comparison of locally made rapid urease test and histology for the diagnosis of Helicobacter pylori infection.

作者信息

Chu K M, Poon R, Tuen H H, Law S Y, Branicki F J, Wong J

机构信息

Department of Surgery, The University of Hong Kong, Queen Mary Hospital, China.

出版信息

Gastrointest Endosc. 1997 Dec;46(6):503-6. doi: 10.1016/s0016-5107(97)70004-x.

DOI:10.1016/s0016-5107(97)70004-x

PMID:9434216

Abstract

BACKGROUND

A number of noncommercial preparations of urease test have been described. The present prospective study evaluated the accuracy of one such preparation for the diagnosis of Helicobacter pylori infection.

METHODS

From February 1996 to November 1996, all patients undergoing elective upper endoscopy in a single endoscopy facility were included. Three antral biopsy specimens were taken. Two specimens were subjected to histologic examination, and one specimen was placed into a "locally made rapid urease test" (LRUT). Results of histologic examinations were taken as standards for comparison. The final result of LRUT was obtained on scrutiny of color changes at 4 hours after the start of the test.

RESULTS

Two thousand three hundred sixteen patients (male/female = 1.5:1) with a mean age of 56.7 +/- 0.4 years were included. Five hundred sixty-two patients (24.3%) had a history of eradication treatment for H. pylori. Nine hundred fifty-three patients (41.1%) were found to be positive for H. pylori on histologic examination. In patients in whom a history of eradication therapy was absent, the sensitivity, specificity, and positive and negative predictive values of the LRUT were 92.8%, 97.6%, 97.5%, and 93.0%, respectively. In patients with a history of eradication treatment, the corresponding figures were 76.1%, 99.6%, 96.2%, and 96.9%.

CONCLUSIONS

The locally made rapid urease test provides a simple, safe, rapid, inexpensive, and accurate test for the diagnosis of H. pylori infection.

摘要

背景

已描述了多种非商业性的尿素酶试验制剂。本前瞻性研究评估了其中一种制剂用于诊断幽门螺杆菌感染的准确性。

方法

1996年2月至1996年11月，纳入了在单一内镜检查机构接受择期上消化道内镜检查的所有患者。采集了三块胃窦活检标本。两块标本进行组织学检查，一块标本放入“自制快速尿素酶试验”（LRUT）中。组织学检查结果作为比较的标准。在试验开始4小时后通过观察颜色变化获得LRUT的最终结果。

结果

纳入了2316例患者（男/女 = 1.5:1），平均年龄为56.7±0.4岁。562例患者（24.3%）有幽门螺杆菌根除治疗史。953例患者（41.1%）经组织学检查发现幽门螺杆菌阳性。在无根除治疗史的患者中，LRUT的敏感性、特异性、阳性预测值和阴性预测值分别为92.8%、97.6%、97.5%和93.0%。在有根除治疗史的患者中，相应的数据分别为76.1%、99.6%、96.2%和96.9%。