Moustapha A, Ross D B, Bittira B, Van-Velzen D, McAlister V C, Lannon C L, Lee T D
Department of Surgery, Dalhousie University, Halifax, Nova Scotia, Canada.
J Thorac Cardiovasc Surg. 1997 Dec;114(6):891-902. doi: 10.1016/S0022-5223(97)70002-3.
The histopathologic changes of fresh rat aortic valve allografts over time and the effect of cryopreservation were examined.
Fifty-six syngeneic and allogeneic rat aortic valves were transplanted, either fresh or after cryopreservation, and then at different time points they were explanted and histologically examined in a blinded fashion.
Histopathologic changes in the first week are similar in syngeneic and allogeneic grafts. Fresh syngeneic grafts and leaflets retained normal structure up to 56 days. Allogeneic grafts showed retrovalvular thrombus formation with leaflet ghosts and neointimal proliferation. Cryopreservation did not alter this process.
Cardiac allograft valves in the rat model undergo changes that are characteristic of cell-mediated rejection and lead to valve failure.
研究新鲜大鼠主动脉瓣同种异体移植随时间推移的组织病理学变化以及冷冻保存的影响。
将56个同基因和同种异体大鼠主动脉瓣进行移植，分为新鲜移植组和冷冻保存后移植组，然后在不同时间点取出并进行盲法组织学检查。
同基因和同种异体移植物在第一周的组织病理学变化相似。新鲜同基因移植物和瓣叶在长达56天内保持正常结构。同种异体移植物显示瓣周血栓形成伴瓣叶残影和内膜增生。冷冻保存未改变这一过程。
大鼠模型中的心脏同种异体移植瓣膜会发生细胞介导排斥反应的特征性变化，并导致瓣膜功能衰竭。
