Paias F O, Lanchote V L, Takayanagui O M, Bonato P S
Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-USP, Brazil.
Chirality. 1997;9(8):722-6. doi: 10.1002/(SICI)1520-636X(1997)9:8<722::AID-CHIR2>3.0.CO;2-7.
We present a method for the enantioselective analysis of albendazole sulfoxide (ABZSO) in plasma for application in clinical pharmacokinetic studies. ABZSO enantiomers were separated on a 5-micron Chiralcel OB-H column (4.6 x 150 mm) using hexane:ethanol (93:7, v/v) as the mobile phase and fluorescence detection. ABZSO was extracted with chloroform:isopropanol (8:2, v/v) from 500-microliter aliquots of acidified plasma, with full drug recovery. The proposed method presented quantitation limits of 20 ng/ml for (-)ABZSO and 50 ng/ml for (+)ABZSO and was linear up to a concentration of 5,000 ng/ml of each enantiomer.
