齿垢密螺旋体mcpA（一种假定的化学感受器基因）的鉴定、测序及表达

Identification, sequence, and expression of Treponema denticola mcpA, a putative chemoreceptor gene.

作者信息

Greene S R, Stamm L V

机构信息

Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599-7400, USA.

出版信息

FEMS Microbiol Lett. 1997 Dec 15;157(2):245-9. doi: 10.1111/j.1574-6968.1997.tb12780.x.

DOI:10.1111/j.1574-6968.1997.tb12780.x

PMID:9435104

Abstract

The nucleotide sequence of a methyl-accepting chemotaxis protein gene, mcpA, from Treponema denticola has been determined. The mcpA gene encodes a 729-amino acid protein whose deduced amino acid sequence has significant homology with several bacterial MCPs. T. denticola McpA contains two N-terminal transmembrane regions and two C-terminal putative methylation sequences that are separated by a highly conserved signaling domain. The organization of these structural features is characteristic of MCPs. The observed molecular mass of the in vitro synthesized McpA (76.0 kDa) correlates with the predicted molecular mass of the protein (80.1 kDa).

摘要

已确定来自齿垢密螺旋体的甲基接受趋化蛋白基因mcpA的核苷酸序列。mcpA基因编码一种729个氨基酸的蛋白质，其推导的氨基酸序列与几种细菌的MCP具有显著同源性。齿垢密螺旋体McpA包含两个N端跨膜区域和两个C端假定的甲基化序列，它们被一个高度保守的信号域隔开。这些结构特征的组织是MCP的特征。体外合成的McpA的观察分子量（76.0 kDa）与该蛋白质的预测分子量（80.1 kDa）相关。