Haramaki M, Yano H, Iemura A, Momosaki S, Ogasawara S, Inoue M, Yamaguchi R, Kusaba A, Utsunomiya I, Kojiro M
First Department of Pathology, Kurume University School of Medicine.
Hum Cell. 1997 Sep;10(3):183-92.
We established a new human hepatocellular carcinoma (HCC) cell line, designated HAK-2, from a surgically resected HCC of a 57-yr-old Japanese man. The patient's tumor consisted of 5 different histological features in a single nodule: well-differentiated HCC with trabecular pattern; and moderately differentiated HCC with 4 different patterns (i.e., trabecular, pseudoglandular, solid, and scirrhous). Morphologically, HAK-2 cells on a plastic dish showed oval-shaped nuclei and large flat, polygonal eosinophilic cytoplasm and proliferated in a monolayered sheet with a population doubling time of 36.8 hours. Meanwhile, various structures, such as compact, trabecular, and tubular arrangements, were induced in HAK-2 cells cultured in type I collagen gel matrix. Also, HAK-2 cells in vitro underwent spontaneous apoptosis more frequently than other HCC cell lines examined. HAK-2 cells secreted various plasma proteins including albumin into the culture medium. Chromosome and flow cytometric analyses revealed that HAK-2 had many structural abnormalities with human karyotype and a single aneuploid cell population with a DNA index of 3.7, respectively. These findings suggest that HAK-2 is a new human HCC cell line representing two morphological characteristics; (1) formation of various structures in the presence of extracellular matrix and (2) frequent spontaneous apoptosis in vitro.
我们从一名57岁日本男性手术切除的肝细胞癌（HCC）中建立了一种新的人肝癌细胞系，命名为HAK - 2。患者的肿瘤在单个结节中具有5种不同的组织学特征：具有小梁状模式的高分化HCC；以及具有4种不同模式（即小梁状、假腺管状、实性和硬化性）的中分化HCC。在塑料培养皿上，HAK - 2细胞形态上表现为椭圆形细胞核以及大的扁平、多边形嗜酸性细胞质，并以单层片状增殖，群体倍增时间为36.8小时。同时，在I型胶原凝胶基质中培养的HAK - 2细胞诱导形成了各种结构，如紧密排列、小梁状和管状排列。此外，与所检测的其他肝癌细胞系相比，HAK - 2细胞在体外更频繁地发生自发凋亡。HAK - 2细胞向培养基中分泌包括白蛋白在内的各种血浆蛋白。染色体和流式细胞术分析显示，HAK - 2分别具有许多人类核型的结构异常和一个DNA指数为3.7的单倍体细胞群体。这些发现表明，HAK - 2是一种新的人肝癌细胞系，代表两种形态学特征：（1）在细胞外基质存在下形成各种结构；（2）在体外频繁发生自发凋亡。
